Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Saitohin (STH) Standard Curve.)
Human Saitohin (STH) ELISA Kit | STH elisa kit
Human Saitohin (STH) ELISA Kit
Gene Names
STH; MAPTIT
Reactivity
Human
Synonyms
Saitohin (STH); Human Saitohin (STH) ELISA Kit; STH; MAPTIT; MGC163191; MGC163193; microtubule-associated protein tau (MAPT) intronic transcript; STH elisa kit
Reactivity
Human
Specificity
Human Saitohin (STH) ELISA Kit has high sensitivity and excellent specificity for detection of Human STH. No significant cross-reactivity or interference between Human STH and analogues was observed.
Sequence Length
128
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Saitohin (STH) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Saitohin (STH) Standard Curve.)
Related Product Information for STH elisa kit
Description: This Human Saitohin (STH) ELISA Kit employs a two-site sandwich ELISA to quantitate STH in samples. An antibody specific for STH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any STH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: During the examination of ESTs in the human tau locus, Conrad et al. (2002) identified a gene they designated saitohin, or STH, in honor of the late Dr. Tsuanao Saitoh and his laboratory. The STH gene encodes a predicted 128-amino acid protein.highest expression of STH was in placenta, muscle, fetal brain, and adult brain, with lower expression in heart, kidney, stomach, testis, and adrenal gland. In the central nervous system, highest expression was in temporal lobe, hypothalamus, medulla, and spinal cord, with lower expression in other brain regions. There was significant overlap with the general tissue and CNS expression of tau, although there were some differences. The results indicated that STH is not under the regulation of the tau promoter, but may share some regional regulatory elements with the tau gene.
Background: During the examination of ESTs in the human tau locus, Conrad et al. (2002) identified a gene they designated saitohin, or STH, in honor of the late Dr. Tsuanao Saitoh and his laboratory. The STH gene encodes a predicted 128-amino acid protein.highest expression of STH was in placenta, muscle, fetal brain, and adult brain, with lower expression in heart, kidney, stomach, testis, and adrenal gland. In the central nervous system, highest expression was in temporal lobe, hypothalamus, medulla, and spinal cord, with lower expression in other brain regions. There was significant overlap with the general tissue and CNS expression of tau, although there were some differences. The results indicated that STH is not under the regulation of the tau promoter, but may share some regional regulatory elements with the tau gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
saitohin
NCBI Official Synonym Full Names
saitohin
NCBI Official Symbol
STH
NCBI Official Synonym Symbols
MAPTIT
NCBI Protein Information
saitohin
UniProt Protein Name
Saitohin
Protein Family
UniProt Gene Name
STH
Uniprot Description
Enzyme and pathway databasesBioCyc Collection of Pathway/Genome DatabasesMore...BioCyciZFISH:G66-32837-MONOMER.
Research Articles on STH
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Product Notes
The Human STH sth (Catalog #AAA9714052) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714052 ELISA Kit recognizes Human STH. It is sometimes possible for the material contained within the vial of "Saitohin (STH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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