Human S100 Calcium Binding Protein A14 (S100A14) ELISA Kit | S100A14 elisa kit

Human S100 Calcium Binding Protein A14 (S100A14) ELISA Kit

Cat. Num. AAA452287

• Gene Names: S100A14; BCMP84; S100A15
• Reactivity: Human
• Synonyms: S100 Calcium Binding Protein A14 (S100A14); Human S100 Calcium Binding Protein A14 (S100A14) ELISA Kit; S100-A14; S100-A15; S100A15; BCMP84; S114; S100A14 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of S100A14. No significant cross-reactivity or interference between S100A14 and analogues was observed.
• Sequence Length: 104

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.102ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100A14 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100A14 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100A14 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of S100A14 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100A14. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to S100A14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100A14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of S100A14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10190712
• NCBI GeneID: 57402
• NCBI Accession #: NP_065723.1
• NCBI GenBank Nucleotide #: NM_020672.2
• UniProt Accession #: Q9HCY8; Q5RHT0
• Molecular Weight: 11,662 Da
• NCBI Official Full Name: protein S100-A14
• NCBI Official Synonym Full Names: S100 calcium binding protein A14
• NCBI Official Symbol: S100A14
• NCBI Official Synonym Symbols: BCMP84; S100A15
• NCBI Protein Information: protein S100-A14
• UniProt Protein Name: Protein S100-A14
• Protein Family: Protein
• UniProt Gene Name: S100A14
• UniProt Synonym Gene Names: S100A15; S114

NCBI Description

This gene encodes a member of the S100 protein family which contains an EF-hand motif and binds calcium. The gene is located in a cluster of S100 genes on chromosome 1. Levels of the encoded protein have been found to be lower in cancerous tissue and associated with metastasis suggesting a tumor suppressor function (PMID: 19956863, 19351828). [provided by RefSeq, Dec 2011]

Uniprot Description

Modulates P53/TP53 protein levels, and thereby plays a role in the regulation of cell survival and apoptosis. Depending on the context, it can promote cell proliferation or apoptosis. Plays a role in the regulation of cell migration by modulating the levels of MMP2, a matrix protease that is under transcriptional control of P53/TP53. Does not bind calcium.