Human S100 Calcium Binding Protein A11 (S100A11) ELISA Kit | S100A11 elisa kit

Human S100 Calcium Binding Protein A11 (S100A11) ELISA Kit

Cat. Num. AAA2022702

• Gene Names: S100A11; MLN70; S100C; HEL-S-43
• Reactivity: Human
• Synonyms: S100 Calcium Binding Protein A11 (S100A11); Human S100 Calcium Binding Protein A11 (S100A11) ELISA Kit; S100-A11; S100C; MLN70; Protein S100-C; Calgizzarin; Metastatic lymph node gene 70 protein; Protein S100-A11; N-terminally processed; S100A11 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A11 (S100A11).; No significant cross-reactivity or interference between S100 Calcium Binding Protein A11 (S100A11) and analogues was observed.
• Sequence Length: 105

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 6.7pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein A11 (S100A11) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein A11 (S100A11) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100A11 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A11 (S100A11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A11 (S100A11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A11 (S100A11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A11 (S100A11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for S100A11 elisa kit

Tumor immunity; Infection immunity; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 15680066
• NCBI GeneID: 6282
• UniProt Accession #: P31949; Q5VTK0
• Molecular Weight: 11,740 Da
• NCBI Official Full Name: S100 calcium binding protein A11
• NCBI Official Synonym Full Names: S100 calcium binding protein A11
• NCBI Official Symbol: S100A11
• NCBI Official Synonym Symbols: MLN70; S100C; HEL-S-43
• NCBI Protein Information: protein S100-A11
• UniProt Protein Name: Protein S100-A11
• Protein Family: Protein
• UniProt Gene Name: S100A11
• UniProt Synonym Gene Names: MLN70; S100C; MLN 70
• UniProt Entry Name: S10AB_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A11: a calcium-binding regulatory protein of the S-100 family. Interacts with the N-termini of annexins A1. May regulate fusion processes such as endo- and exocytosis. Increases in the early stage of pancreatic carcinogenesis and decreases during subsequent progression to cancer. Increased expression in anaplastic large cell lymphoma.

Protein type: DNA replication; Cell cycle regulation; Calcium-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; ruffle; extracellular space; cytoplasm; nucleus

Molecular Function: protein binding; protein homodimerization activity; calcium ion binding; calcium-dependent protein binding

Biological Process: negative regulation of cell proliferation; negative regulation of DNA replication; signal transduction