Human S100-B ELISA Kit | S100-B elisa kit

Human Protein S100-B ELISA Kit

Cat. Num. AAA9425800

• Gene Names: S100B; NEF; S100; S100-B; S100beta
• Reactivity: Human
• Synonyms: S100-B; Human Protein S100-B ELISA Kit; Protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; S100B; S100-B elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human PDIA4. No significant cross-reactivity or interference was observed.

• Samples: Serum, plasma, tissue homogenates and cell culture supernates and other biological fluids
• Detection Range: 15.6 pg/ml - 1000 pg/ml
• Sensitivity: less than7.8pg/ml
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100-B elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Protein S100-B concentrations in serum, plasma, tissue homogenates and cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein S100-B. Standards or samples are then added to the appropriate microtiter plate wells with a biotinconjugated polyclonal antibody preparation specific for Protein S100-B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein S100-B, biotin-conjugated antibody and enzymeconjugated Avidin will exhibit a change in color. The enzymesubstrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Protein S100-B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5454034
• NCBI GeneID: 6285
• NCBI Accession #: NP_006263.1
• NCBI GenBank Nucleotide #: NM_006272.2
• UniProt Accession #: P04271; D3DSN6
• Molecular Weight: 10,713 Da
• NCBI Official Full Name: protein S100-B
• NCBI Official Synonym Full Names: S100 calcium binding protein B
• NCBI Official Symbol: S100B
• NCBI Official Synonym Symbols: NEF; S100; S100-B; S100beta
• NCBI Protein Information: protein S100-B
• UniProt Protein Name: Protein S100-B
• UniProt Gene Name: S100B

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

Uniprot Description

S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytoplasm; extracellular region; nucleus; perinuclear region of cytoplasm; ruffle

Molecular Function: calcium-dependent protein binding; identical protein binding; protein binding; protein homodimerization activity; RAGE receptor binding; tau protein binding; zinc ion binding

Biological Process: axonogenesis; cell proliferation; central nervous system development; innate immune response; learning and/or memory; positive regulation of I-kappaB kinase/NF-kappaB cascade