Human Rubeola virus IgG ELISA Kit
Human Rubeola virus IgG ELISA Kit
Reactivity
Human
Applications
ELISA
Synonyms
Rubeola virus IgG; Human Rubeola virus IgG ELISA Kit; Measles ELISA Kit; IgG ELISA Kit; Rubeola virus ELISA Kit; Rubeola virus IgG elisa kit
Reactivity
Human
Applicable Applications for Rubeola virus IgG elisa kit
ELISA (EIA)
Application Notes
The Measles IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Measles antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Measles antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti human-IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color.
Biological Significance
Measles is a highly contagious viral disease characterized by a clinically distinct prodrome of fever, coryza, conjunctivitis, cough and a pathognomic exanthem (Koplik`s spots). The disease is the result of infection with the Measles Virus, genus Morbillivirus of the family Paramyxoviridae.
Biohazard Information
All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.
Preparation and Storage
Store at 4 degree C.
Related Product Information for Rubeola virus IgG elisa kit
ELISA kit for detection of Rubeola virus IgG in the research laboratory
Product Categories/Family for Rubeola virus IgG elisa kit
Similar Products
Product Notes
The Human Rubeola virus IgG (Catalog #AAA539700) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA539700 ELISA Kit recognizes Human Rubeola virus IgG. AAA Biotech's Rubeola virus IgG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). The Measles IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Measles antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Measles antigen takes place. After an one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti human-IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color. Researchers should empirically determine the suitability of the Rubeola virus IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rubeola virus IgG, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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