Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Retinol-binding protein 1 (RBP1) Standard Curve.)
Human Retinol-Binding Protein 1 (RBP1) ELISA Kit | RBP1 elisa kit
Human Retinol-Binding Protein 1 (RBP1) ELISA Kit
Gene Names
RBP1; CRBP1
Reactivity
Human
Synonyms
Retinol-Binding Protein 1 (RBP1); Human Retinol-Binding Protein 1 (RBP1) ELISA Kit; RBP1; CRABP-I; CRBP; CRBP1; CRBPI; RBPC; retinol-binding protein 1; cellular; RBP1 elisa kit
Reactivity
Human
Specificity
Human Retinol-binding protein 1 (RBP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human RBP1. No significant cross-reactivity or interference between Human RBP1 and analogues was observed.
Sequence Length
135
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Retinol-binding protein 1 (RBP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Retinol-binding protein 1 (RBP1) Standard Curve.)
Related Product Information for RBP1 elisa kit
Description: This Human Retinol-binding protein 1 (RBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate RBP1 in samples. An antibody specific for RBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: RBP1 is the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. The gene harbors four exons encoding 24, 59, 33, and 16 amino acid residues respectively. The second intervening sequence alone occupies 19 kb of the 21 kb of the gene. Retinol-binding protein-4 (RBP4) is the carrier protein involved in the transport of retinol from the liver storage site to peripheral tissue. On the other hand, cellular RBP (CRBP) is the intracellular carrier involved in intracellular movement of retinol.Colantuoni et al. (1985) isolated and sequenced a cDNA clone for the human CRBP gene.
Background: RBP1 is the carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. The gene harbors four exons encoding 24, 59, 33, and 16 amino acid residues respectively. The second intervening sequence alone occupies 19 kb of the 21 kb of the gene. Retinol-binding protein-4 (RBP4) is the carrier protein involved in the transport of retinol from the liver storage site to peripheral tissue. On the other hand, cellular RBP (CRBP) is the intracellular carrier involved in intracellular movement of retinol.Colantuoni et al. (1985) isolated and sequenced a cDNA clone for the human CRBP gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
retinol-binding protein 1
NCBI Official Synonym Full Names
retinol binding protein 1
NCBI Official Symbol
RBP1
NCBI Official Synonym Symbols
CRBP1
NCBI Protein Information
retinol-binding protein 1
UniProt Protein Name
Retinol-binding protein 1
Protein Family
UniProt Gene Name
RBP1
UniProt Synonym Gene Names
CRBP; CRBP-I
UniProt Entry Name
RET1_BOVIN
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Product Notes
The Human RBP1 rbp1 (Catalog #AAA9714485) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714485 ELISA Kit recognizes Human RBP1. It is sometimes possible for the material contained within the vial of "Retinol-Binding Protein 1 (RBP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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