Human RB1 ELISA Kit | RB1 elisa kit

Human RB1 (Retinoblastoma Protein 1) ELISA Kit

Cat. Num. AAA2509425

• Gene Names: RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
• Reactivity: Human
• Synonyms: RB1; Human RB1 (Retinoblastoma Protein 1) ELISA Kit; RB1 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human RB1 in samples. No significant cross-reactivity or interference between Human RB1 and analogues was observed.
• Sequence Length: 928

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.16-10ng/mL
• Sensitivity: 0.1ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human RB1 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human RB1 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RB1 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human RB1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RB1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RB1 and Avidin- Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RB1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human RB1. You can calculate the concentration of Human RB1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 108773787
• NCBI GeneID: 5925
• NCBI Accession #: NP_000312.2
• NCBI GenBank Nucleotide #: NM_000321.2
• UniProt Accession #: P06400; P78499; Q5VW46; Q8IZL4; A8K5E3
• Molecular Weight: 106,159 Da
• NCBI Official Full Name: retinoblastoma-associated protein
• NCBI Official Synonym Full Names: retinoblastoma 1
• NCBI Official Symbol: RB1
• NCBI Official Synonym Symbols: RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
• NCBI Protein Information: retinoblastoma-associated protein; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; retinoblastoma suspectibility protein
• UniProt Protein Name: Retinoblastoma-associated protein
• Protein Family: RB1-inducible coiled-coil protein
• UniProt Gene Name: RB1
• UniProt Synonym Gene Names: Rb
• UniProt Entry Name: RB_HUMAN

NCBI Description

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Uniprot Description

Rb: retinoblastoma tumor suppressor protein regulates cell proliferation by controlling progression through the G1-phase restriction point. Has three distinct binding domains and interacts with regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by Cdks inhibits Rb target binding, thus allowing cell cycle progression. Recruits and targets histone methyltransferase suv39h1 leading to epigenetic transcriptional repression.

Protein type: Nuclear receptor co-regulator; Tumor suppressor; Oncoprotein; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 13q14.2

Cellular Component: nucleoplasm; SWI/SNF complex; PML body; Rb-E2F complex; spindle; chromatin; nucleus

Molecular Function: identical protein binding; protein binding; androgen receptor binding; DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; phosphoprotein binding; kinase binding; transcription factor binding; transcription factor activity

Biological Process: viral reproduction; sister chromatid biorientation; negative regulation of transcription from RNA polymerase II promoter, mitotic; positive regulation of transcription, DNA-dependent; regulation of mitotic cell cycle; neuron maturation; cell cycle arrest; positive regulation of macrophage differentiation; neurite development; negative regulation of epithelial cell proliferation; transcription, DNA-dependent; negative regulation of transcription factor activity; mitotic cell cycle checkpoint; enucleate erythrocyte differentiation; regulation of lipid kinase activity; chromatin remodeling; myoblast differentiation; G1/S-specific transcription in mitotic cell cycle; neuron apoptosis; maintenance of mitotic sister chromatid cohesion; gut development; cell division; neuron morphogenesis during differentiation; negative regulation of smoothened signaling pathway; Ras protein signal transduction; androgen receptor signaling pathway; positive regulation of mitotic metaphase/anaphase transition; negative regulation of protein kinase activity; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; cell cycle checkpoint; G1/S transition of mitotic cell cycle

Disease: Bladder Cancer; Osteogenic Sarcoma; Small Cell Cancer Of The Lung; Retinoblastoma