TACI Recombinant Protein | TNFRSF13B recombinant protein

TACI Protein, Human, Recombinant (ECD, hFc Tag)

Cat. Num. AAA8118693

• Gene Names: TNFRSF13B; CVID; RYZN; TACI; CD267; CVID2; IGAD2; TNFRSF14B
• Purity: >90 % as determined by SDS-PAGE.
• Synonyms: TACI; TACI Protein; Human; Recombinant (ECD; hFc Tag); Human TACI/TNFRSF13B Protein (ECD; Fc Tag); tumor necrosis factor receptor superfamily; member 13B; CD267 Protein; CVID Protein; CVID2 Protein; IGAD2 Protein; RYZN Protein; TNFRSF14B Protein; TNFRSF13B recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 403aa
• Sequence: Ser2-Thr166

• Species: Human
• Tag: hFc
• Endotoxin: <1.0EU per ug protein as determined by the LAL method
• Predicted N Terminal: Ser2
• Protein Construction: |A DNA sequence encoding the human TNFRSF13B (NP_036584.1) (Ser2-Thr166) was expressed with the Fc region of human IgG1 at the C-terminus
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human S3-TNFRSF13B-Fc at 2ug/ml (100ul/well) can bind human Fc-BAFF, Biotinylated. The EC50 of human BAFF, Biotinylated is 1.5-5.5ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human S3-TNFRSF13B-Fc at 2 ug/ml (100 ul/well) can bind human Fc-BAFF, Biotinylated, the EC50 of human BAFF, Biotinylated is 1.5-5.5 ng/mL.)

Related Product Information for TNFRSF13B recombinant protein

Tumor necrosis factor receptor superfamily, member 13B (TNFRSF13B) also known as Transmembrane activator and CAML interactor (TACI) and CD267 antigen, is a member of the tumor necrosis factor receptor superfamily. TNFRSF13B is a trimeric cytokine receptor that binds tumor necrosis factors (TNF). The receptor cooperates with an adaptor protein which is important in determining the outcome of the response. Members of the TNF receptor superfamily (TNFRSF) have crucial roles in both innate and adaptive immunity and in cellular apoptosis process. Apoptosis is a cell suicide mechanism that enables metazoans to control cell number in tissues and to eliminate individual cells that threaten the animal's survival. Certain cells have unique sensors, termed death receptors or tumour necrosis factor (TNFR), on their surface. Tumour necrosis factors (TNFR) detect the presence of extracellular death signals and, in response, they rapidly ignite the cell's intrinsic apoptosis machinery. TACI/TNFRSF13B/CD267 induces activation of the transcription factors NFAT, AP1, and NF-kappa-B and plays a crucial role in humoral immunity by interacting with a TNF ligand.

References

Salzer U, et al. (2005) Mutations in TNFRSF13B encoding TACI are associated with common variable immunodeficiency in humans. Nat Genet. 37(8): 820-8.Salzer U, et al. (2009) Relevance of biallelic versus monoallelic TNFRSF13B mutations in distinguishing disease-causing from risk-increasing TNFRSF13B variants in antibody deficiency syndromes. Blood. 113(9): 1967-76.Mohammadi J, et al. (2009) Novel mutations in TACI (TNFRSF13B) causing common variable immunodeficiency. J Clin Immunol. 29(6): 777-85.

NCBI and Uniprot Product Information

• NCBI GI #: 6912694
• NCBI GeneID: 23495
• NCBI Accession #: NP_036584.1
• NCBI GenBank Nucleotide #: NM_012452.2
• UniProt Accession #: Q4ACX1; O14836
• Molecular Weight: 31,816 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 13B
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 13B
• NCBI Official Symbol: TNFRSF13B
• NCBI Official Synonym Symbols: CVID; RYZN; TACI; CD267; CVID2; IGAD2; TNFRSF14B
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 13B; transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B
• UniProt Protein Name: TACI
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TACI
• UniProt Entry Name: Q4ACX1_HUMAN

NCBI Description

The protein encoded by this gene is a lymphocyte-specific member of the tumor necrosis factor (TNF) receptor superfamily. It interacts with calcium-modulator and cyclophilin ligand (CAML). The protein induces activation of the transcription factors NFAT, AP1, and NF-kappa-B and plays a crucial role in humoral immunity by interacting with a TNF ligand. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF13B: Receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS that binds both ligands with similar high affinity. Mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-kappa-B and AP-1. Involved in the stimulation of B- and T- cell function and the regulation of humoral immunity. Defects in TNFRSF13B are the cause of immunodeficiency common variable type 2 (CVID2). CVID2 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Defects in TNFRSF13B are a cause of immunoglobulin A deficiency 2 (IGAD2). Selective deficiency of immunoglobulin A (IGAD) is the most common form of primary immunodeficiency, with an incidence of approximately 1 in 600 individuals in the western world. Individuals with symptomatic IGAD often have deficiency of IgG subclasses or decreased antibody response to carbohydrate antigens such as pneumococcal polysaccharide vaccine. Individuals with IGAD also suffer from recurrent sinopulmonary and gastrointestinal infections and have an increased incidence of autoimmune disorders and of lymphoid and non-lymphoid malignancies. In vitro studies have suggested that some individuals with IGAD have impaired isotype class switching to IgA and others may have a post-switch defect. IGAD and CVID have been known to coexist in families. Some individuals initially present with IGAD1 and then develop CVID. These observations suggest that some cases of IGAD and CVID may have a common etiology. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Cell cycle regulation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: integral to plasma membrane; external side of plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: cell surface receptor linked signal transduction; B cell homeostasis; hemopoietic progenitor cell differentiation; negative regulation of B cell proliferation

Disease: Immunodeficiency, Common Variable, 2; Immunoglobulin A Deficiency 2