Human Ras protein ELISA Kit | Ras elisa kit

Human Ras protein ELISA Kit

Cat. Num. AAA745819

• Gene Names: HRAS; CTLO; HAMSV; HRAS1; RASH1; p21ras; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1
• Reactivity: Human
• Synonyms: Ras protein; Human Ras protein ELISA Kit; Ras elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 10-250ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This Ras protein ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Ras protein. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Ras elisa kit

Principle of the assay: Ras protein ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Ras protein antibody and an Ras protein-HRP conjugate. The assay sample and buffer are incubated together with Ras protein-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Ras protein concentration since Ras protein from samples and Ras protein-HRP conjugate compete for the anti-Ras protein antibody binding site. Since the number of sites is limited, as more sites are occupied by Ras protein from the sample, fewer sites are left to bind Ras protein-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ras protein concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 190938
• NCBI GeneID: 3265
• Molecular Weight: 18,870 Da
• NCBI Official Full Name: ras protein, partial
• NCBI Official Synonym Full Names: Harvey rat sarcoma viral oncogene homolog
• NCBI Official Symbol: HRAS
• NCBI Official Synonym Symbols: CTLO; HAMSV; HRAS1; RASH1; p21ras; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1
• NCBI Protein Information: GTPase HRas; p19 H-RasIDX protein; c-has/bas p21 protein; transforming protein p21; Ha-Ras1 proto-oncoprotein; c-ras-Ki-2 activated oncogene; GTP- and GDP-binding peptide B; transformation gene: oncogene HAMSV; Harvey rat sarcoma viral oncoprotein; Ras family small GTP binding protein H-Ras; v-Ha-ras Harvey rat sarcoma viral oncogene homolog
• UniProt Protein Name: GTPase HRas
• Protein Family: Ras protein
• UniProt Gene Name: HRAS
• UniProt Synonym Gene Names: HRAS1
• UniProt Entry Name: RASH_HUMAN

NCBI Description

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HRas: a small GTPase protein of the Ras family. Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Mutations are implicated in a variety of human tumors.

Protein type: G protein, monomeric; Motility/polarity/chemotaxis; G protein, monomeric, Ras; G protein; Oncoprotein

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: Golgi membrane; Golgi apparatus; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: protein C-terminus binding; protein binding; GTP binding

Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; activation of MAPKK activity; nerve growth factor receptor signaling pathway; protein heterooligomerization; positive regulation of JNK cascade; chemotaxis; signal transduction; positive regulation of MAP kinase activity; negative regulation of cell proliferation; synaptic transmission; cell surface receptor linked signal transduction; positive regulation of MAPKKK cascade; small GTPase mediated signal transduction; positive regulation of cell proliferation; ephrin receptor signaling pathway; visual learning; negative regulation of neuron apoptosis; cell cycle arrest; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; mitotic cell cycle checkpoint; MAPKKK cascade; endocytosis; social behavior; regulation of synaptic transmission, GABAergic; cell proliferation; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; organ morphogenesis; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; blood coagulation; actin cytoskeleton organization and biogenesis; leukocyte migration; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; positive regulation of cell migration

Disease: Bladder Cancer; Thyroid Carcinoma, Follicular; Costello Syndrome; Schimmelpenning-feuerstein-mims Syndrome; Melanocytic Nevus Syndrome, Congenital; Nevus, Epidermal