Rabbit PHO1 Polyclonal Antibody | anti-PHO1 antibody

PHO1 Polyclonal Antibody

Cat. Num. AAA3005679

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PHO1; Polyclonal Antibody; PHO1 Polyclonal Antibody; DNA dC->dU-editing enzyme APOBEC-3A; A3A; Phorbolin-1; APOBEC3A; Phorbolin 1; Phorbolin1; PHO-1; anti-PHO1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PHO1 polyclonal antibody detects endogenous levels of PHO1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 199

• Applicable Applications for anti-PHO1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 67-112 of Human PHO1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PHO1 polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate)

Related Product Information for anti-PHO1 antibody

APOBEC3A (apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A), also known as PHO1 or ARP3 or PHRBN is a 199 amino acid protein that belongs to the cytidine deaminase family. Expressed in keratinocytes, as well as in peripherical leukocytes, APOBEC3A may be implicated in cell growth and cell cycle control. The gene encoding APOBEC3A is one of seven related genes which map to a cluster on chromosome 22 and are thought to play a role in tumor transformation and metastasis, as well as in host-associated antiviral activity. Chromosome 22 houses over 500 genes, some of which are involved in Phelan-McDermid syndrome, schizophrenia and Neurofibromatosis type 2.

NCBI and Uniprot Product Information

• NCBI GI #: 300869638
• NCBI Accession #: NP_001180218.1
• NCBI GenBank Nucleotide #: NP_001180218.1
• UniProt Accession #: P31941
• Molecular Weight: ~ 23kDa
• NCBI Official Full Name: probable DNA dC->dU-editing enzyme APOBEC-3A
• UniProt Protein Name: DNA dC->dU-editing enzyme APOBEC-3A
• Protein Family: Phosphate transporter
• UniProt Gene Name: APOBEC3A
• UniProt Synonym Gene Names: A3A
• UniProt Entry Name: ABC3A_HUMAN

Uniprot Description

APOBEC3A: a single-stranded DNA cytidine deaminase involved in foreign DNA clearance with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA. Can induce somatic C-to-U hypermutation in nuclear and mitochondrial DNA. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation. Interacts with AGO2. Interacts with TRIB3. Expressed in peripheral leukocytes with higher expression in CD14-positive phagocytic cells. Highly expressed in keratinocytes and in periphery blood monocytes. Also detected in non-lymphoid tissues including lung and adipose tissues. Found at high levels in colorectal adenocarcinoma, Burkitt's lymphoma and chronic myelogenous leukemia. Up-regulated by interferon and CpG single-stranded DNA (at protein level). Belongs to the cytidine and deoxycytidylate deaminase family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 3.5.4.-; Hydrolase

Chromosomal Location of Human Ortholog: 22q13.1-q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; deoxycytidine deaminase activity; zinc ion binding; cytidine deaminase activity

Biological Process: negative regulation of viral genome replication; innate immune response; cytidine deamination; defense response to virus