Human RalA Binding Protein 1 (RALBP1) ELISA Kit | RALBP1 elisa kit

Human RalA Binding Protein 1 (RALBP1) ELISA Kit

Cat. Num. AAA2023844

• Gene Names: RALBP1; RIP1; RLIP1; RLIP76
• Reactivity: Human
• Synonyms: RalA Binding Protein 1 (RALBP1); Human RalA Binding Protein 1 (RALBP1) ELISA Kit; RIP; RIP1; RLIP76; 76 kDa Ral-interacting protein; Ral-interacting protein 1; Dinitrophenyl S-glutathione ATPase; RALBP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RalA Binding Protein 1 (RALBP1).; No significant cross-reactivity or interference between RalA Binding Protein 1 (RALBP1) and analogues was observed.
• Sequence Length: 655

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RalA Binding Protein 1 (RALBP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RalA Binding Protein 1 (RALBP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RALBP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RalA Binding Protein 1 (RALBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RalA Binding Protein 1 (RALBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RalA Binding Protein 1 (RALBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RalA Binding Protein 1 (RALBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for RALBP1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 5803145
• NCBI GeneID: 10928
• NCBI Accession #: NP_006779.1
• NCBI GenBank Nucleotide #: NM_006788.3
• UniProt Accession #: Q15311; D3DUI0
• Molecular Weight: 76,063 Da
• NCBI Official Full Name: ralA-binding protein 1
• NCBI Official Synonym Full Names: ralA binding protein 1
• NCBI Official Symbol: RALBP1
• NCBI Official Synonym Symbols: RIP1; RLIP1; RLIP76
• NCBI Protein Information: ralA-binding protein 1
• UniProt Protein Name: RalA-binding protein 1
• Protein Family: RalA-binding protein
• UniProt Gene Name: RALBP1
• UniProt Synonym Gene Names: RLIP1; RLIP76; RalBP1; DNP-SG ATPase
• UniProt Entry Name: RBP1_HUMAN

NCBI Description

RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).[supplied by OMIM, Nov 2010]

Uniprot Description

RALBP1: Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin. Interacts with the GTP-bound form of RALA, RALB, CDC42 and RAC1. Interacts with REPS1 and REPS2 and this does not affect the Ral-binding activity. Interacts with DAB2IP. Interacts with catalytically active CCNB1 and CDK1 during mitosis. Interacts with EPN1, NUMB and TFAP2A during interphase and mitosis. Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.

Protein type: GAPs, Rac/Rho; GAPs

Chromosomal Location of Human Ortholog: 18p11.3

Cellular Component: membrane; cytosol

Molecular Function: protein binding; ATPase activity, coupled to movement of substances; ATPase activity; Ral GTPase binding; Rac GTPase binding; GTPase activator activity

Biological Process: regulation of small GTPase mediated signal transduction; regulation of GTPase activity; metabolic process; transport; small GTPase mediated signal transduction; signal transduction; chemotaxis