Human RalA Binding Protein 1 (RALBP1) ELISA Kit | RALBP1 elisa kit

Human RalA Binding Protein 1 (RALBP1) ELISA Kit

Cat. Num. AAA161724

• Gene Names: RALBP1; RIP1; RLIP1; RLIP76
• Reactivity: Human
• Synonyms: RalA Binding Protein 1 (RALBP1); Human RalA Binding Protein 1 (RALBP1) ELISA Kit; RALBP1 elisa kit

• Reactivity: Human
• Sequence Length: 655

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.05ng/ml - 40ng/ml
• Sensitivity: 0.03ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RALBP1 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human RalA Binding Protein 1 (also known as RALBP1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human RALBP1 antibody. RALBP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human RALBP1 Antibody is added and binds to RALBP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated RALBP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human RALBP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 15341887
• NCBI GeneID: 10928
• Molecular Weight: 76,063 Da
• NCBI Official Full Name: RalA binding protein 1
• NCBI Official Synonym Full Names: ralA binding protein 1
• NCBI Official Symbol: RALBP1
• NCBI Official Synonym Symbols: RIP1; RLIP1; RLIP76
• NCBI Protein Information: ralA-binding protein 1
• UniProt Protein Name: RalA-binding protein 1
• Protein Family: RalA-binding protein
• UniProt Gene Name: RALBP1
• UniProt Synonym Gene Names: RLIP1; RLIP76; RalBP1; DNP-SG ATPase

NCBI Description

RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).[supplied by OMIM, Nov 2010]

Uniprot Description

Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.