Human Radical S-Adenosyl Methionine Domain-Containing Protein 2 (RSAD2) ELISA Kit | RSAD2 elisa kit

Human Radical S-Adenosyl Methionine Domain-Containing Protein 2 (RSAD2) ELISA Kit

Cat. Num. AAA9714449

• Gene Names: RSAD2; cig5; vig1; cig33; 2510004L01Rik
• Reactivity: Human
• Synonyms: Radical S-Adenosyl Methionine Domain-Containing Protein 2 (RSAD2); Human Radical S-Adenosyl Methionine Domain-Containing Protein 2 (RSAD2) ELISA Kit; RSAD2; 2510004L01Rik; cig33; cig5; vig1; viperin; RSAD2 elisa kit

• Reactivity: Human
• Specificity: Human Radical S-adenosyl methionine domain-containing protein 2 (RSAD2) ELISA Kit has high sensitivity and excellent specificity for detection of Human RSAD2. No significant cross-reactivity or interference between Human RSAD2 and analogues was observed.
• Sequence Length: 361

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Radical S-adenosyl methionine domain-containing protein 2 (RSAD2) Standard Curve.)

Related Product Information for RSAD2 elisa kit

Description: This Human Radical S-adenosyl methionine domain-containing protein 2 (RSAD2) ELISA Kit employs a two-site sandwich ELISA to quantitate RSAD2 in samples. An antibody specific for RSAD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RSAD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RSAD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RSAD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The deduced 361-amino acid protein has a calculated molecular mass of 42.1 kD. Viperin contains an N-terminal leucine zipper motif and a domain associated with iron-sulfur cluster coordination. Viperin shares significant homology with Best5, which is expressed during rat osteoblast differentiation, and Vig1, which is induced in rainbow trout infected with a fish rhabdovirus. The N-terminal leucine zipper motifs display the least homology. Western blot analysis of IFNG-treated macrophages and transfected HeLa cells revealed a major band of 43 kD and a minor band of 35 kD.HCMV infection caused redistribution of the induced viperin from its normal endoplasmic reticulum association, first to the Golgi apparatus and then to cytoplasmic vacuoles containing gB and the HCMV structural protein pp28.

NCBI and Uniprot Product Information

• NCBI GI #: 19923667
• NCBI GeneID: 91543
• NCBI Accession #: NP_542388.2
• NCBI GenBank Nucleotide #: NP_542388.2
• UniProt Accession #: Q8WXG1
• NCBI Official Full Name: radical S-adenosyl methionine domain-containing protein 2
• NCBI Official Synonym Full Names: radical S-adenosyl methionine domain containing 2
• NCBI Official Symbol: RSAD2
• NCBI Official Synonym Symbols: cig5; vig1; cig33; 2510004L01Rik
• NCBI Protein Information: radical S-adenosyl methionine domain-containing protein 2
• UniProt Protein Name: Radical S-adenosyl methionine domain-containing protein 2
• Protein Family: Radical S-adenosyl methionine domain-containing protein
• UniProt Gene Name: RSAD2

Uniprot Description

Interferon-inducible iron-sulfur (4FE-4S) cluster-binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), and human immunodeficiency virus (HIV-1). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphosphate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63'-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T-helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins.