Human RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) ELISA Kit | RAD51 elisa kit

Human DNA repair protein RAD51 homolog 1, RAD51 ELISA Kit

Cat. Num. AAA9321586

• Gene Names: RAD51; RECA; BRCC5; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
• Reactivity: Human
• Synonyms: RAD51 homolog (RecA homolog; E. coli) (S. cerevisiae); Human DNA repair protein RAD51 homolog 1; RAD51 ELISA Kit; Human DNA repair protein RAD51 homolog 1 (RAD51) ELISA kit; BRCC5; HRAD51; HsRad51; HsT16930; RAD51A; RECA; BRCA1/BRCA2-containing complex; subunit 5; DNA repair protein RAD51 homolog 1; RAD51 homolog protein; RecA; E. coli; homolog of; RecA-like protein; recombi; RAD51 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human RAD51 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.625ng/ml-20ng/ml.
• Sensitivity: 0.1ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated RAD51 concentrations in Human serum, plasma and other body fluids. Using Purified Human RAD51 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RAD51 and RAD51 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RAD51 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for RAD51 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated RAD51 concentrations in Human serum, plasma and other body fluids. Using Purified Human RAD51 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RAD51 and RAD51 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RAD51 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 256017143
• NCBI GeneID: 5888
• NCBI Accession #: NP_001157741.1
• NCBI GenBank Nucleotide #: NM_001164269.1
• UniProt Accession #: Q06609; Q6FHX9; Q6ZNA8; Q9BV60; B0FXP0; B2R8T6
• Molecular Weight: 36,780 Da
• NCBI Official Full Name: DNA repair protein RAD51 homolog 1 isoform 2
• NCBI Official Synonym Full Names: RAD51 recombinase
• NCBI Official Symbol: RAD51
• NCBI Official Synonym Symbols: RECA; BRCC5; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
• NCBI Protein Information: DNA repair protein RAD51 homolog 1; RAD51 homolog A; RecA-like protein; recombination protein A; RecA, E. coli, homolog of; BRCA1/BRCA2-containing complex, subunit 5
• UniProt Protein Name: DNA repair protein RAD51 homolog 1
• Protein Family: DNA repair protein
• UniProt Gene Name: RAD51
• UniProt Synonym Gene Names: RAD51A; RECA; HsRAD51; hRAD51
• UniProt Entry Name: RAD51_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Uniprot Description

RAD51: a member of the RAD51 protein family. Involved in the homologous recombination and repair of DNA. Interacts with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. Interacts with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both its intracellular localization and DNA-binding ability. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Defects in RAD51 are associated with breast cancer. Two alternatively spliced isoforms have been reported.

Protein type: DNA repair, damage; Mitochondrial

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: nucleoplasm; nuclear chromosome; lateral element; PML body; condensed nuclear chromosome; mitochondrion; mitochondrial matrix; perinuclear region of cytoplasm; cytoplasm; nucleolus; microtubule organizing center; condensed chromosome; nucleus

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; double-stranded DNA binding; damaged DNA binding; single-stranded DNA binding; single-stranded DNA-dependent ATPase activity; ATP binding

Biological Process: DNA unwinding during replication; meiosis; mitotic recombination; DNA recombinase assembly; double-strand break repair; meiotic recombination; positive regulation of DNA ligation; DNA repair; response to DNA damage stimulus; protein homooligomerization; double-strand break repair via homologous recombination; DNA recombination

Disease: Breast Cancer; Mirror Movements 2