Human RAB7A, Member RAS Oncogene Family (RAB7A) ELISA Kit | RAB7A elisa kit

Human RAB7A, Member RAS Oncogene Family (RAB7A) ELISA Kit

Cat. Num. AAA2706843

• Gene Names: RAB7A; RAB7; PRO2706
• Reactivity: Human
• Synonyms: RAB7A; Member RAS Oncogene Family (RAB7A); Human RAB7A; Member RAS Oncogene Family (RAB7A) ELISA Kit; RAB7; Ras-related protein Rab-7a; RAB7A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RAB7A, Member RAS Oncogene Family (RAB7A).; No significant cross-reactivity or interference between RAB7A, Member RAS Oncogene Family (RAB7A) and analogues was observed.
• Sequence Length: 207

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAB7A, Member RAS Oncogene Family (RAB7A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAB7A, Member RAS Oncogene Family (RAB7A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAB7A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAB7A, Member RAS Oncogene Family (RAB7A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAB7A, Member RAS Oncogene Family (RAB7A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAB7A, Member RAS Oncogene Family (RAB7A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RAB7A, Member RAS Oncogene Family (RAB7A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 60688670
• NCBI GeneID: 7879
• Molecular Weight: 23,490 Da
• NCBI Official Full Name: RAB7A, member RAS oncogene family
• NCBI Official Synonym Full Names: RAB7A, member RAS oncogene family
• NCBI Official Symbol: RAB7A
• NCBI Official Synonym Symbols: RAB7; PRO2706
• NCBI Protein Information: ras-related protein Rab-7a
• UniProt Protein Name: Ras-related protein Rab-7a
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB7A
• UniProt Synonym Gene Names: RAB7

NCBI Description

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008]

Uniprot Description

Rab7: a member of the RAB family of RAS-related GTP-binding proteins, important regulators of vesicular transport and are located in specific intracellular compartments. RAB7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori. Mutations in this protein cause Charcot-Marie-Tooth type 2B neuropathy.

Protein type: G protein; G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: autophagosome membrane; cytosol; endosome membrane; extracellular exosome; Golgi apparatus; late endosome; late endosome membrane; lipid droplet; lysosomal membrane; lysosome; melanosome membrane; phagocytic vesicle; phagocytic vesicle membrane; plasma membrane; pre-autophagosomal structure membrane; retromer complex; secretory granule membrane; terminal bouton

Molecular Function: GDP binding; GTP binding; GTPase activity; protein binding; Rac GTPase binding; retromer complex binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; autophagosome assembly; bone resorption; early endosome to late endosome transport; endocytosis; endosome to lysosome transport; epidermal growth factor catabolic process; lipid catabolic process; lipophagy; multi-organism intracellular transport; negative regulation of intralumenal vesicle formation; neutrophil degranulation; phagosome acidification; phagosome maturation; phagosome-lysosome fusion; positive regulation of protein catabolic process; positive regulation of viral reproduction; protein targeting to lysosome; protein to membrane docking; protein transport; release of virus from host; retrograde transport, endosome to Golgi

Disease: Charcot-marie-tooth Disease, Axonal, Type 2b