Human Pumilio homolog 1 (PUM1) ELISA Kit | PUM1 elisa kit

Human Pumilio homolog 1 (PUM1) ELISA Kit

Cat. Num. AAA167463

• Gene Names: PUM1; PUMH; HSPUM; PUMH1; PUML1; SCA47
• Reactivity: Human
• Synonyms: Pumilio homolog 1 (PUM1); Human Pumilio homolog 1 (PUM1) ELISA Kit; PUM1 elisa kit

• Reactivity: Human
• Sequence Length: 1188

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 15ng/L - 3500ng/L
• Sensitivity: 7.82ng/L
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PUM1 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Pumilio Homolog 1 (also known as PUM1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human PUM1 antibody. PUM1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human PUM1 Antibody is added and binds to PUM1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PUM1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human PUM1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 66932909
• NCBI GeneID: 9698
• NCBI Accession #: NP_001018494.1
• NCBI GenBank Nucleotide #: NM_001020658.1
• UniProt Accession #: Q14671; Q53HH5; Q5VXY7; Q9HAN1; A8K6W4; B4DG92; D3DPN3; E9PCJ0
• Molecular Weight: 124,420 Da
• NCBI Official Full Name: pumilio homolog 1 isoform 1
• NCBI Official Synonym Full Names: pumilio RNA binding family member 1
• NCBI Official Symbol: PUM1
• NCBI Official Synonym Symbols: PUMH; HSPUM; PUMH1; PUML1; SCA47
• NCBI Protein Information: pumilio homolog 1
• UniProt Protein Name: Pumilio homolog 1
• Protein Family: Pumilio
• UniProt Gene Name: PUM1
• UniProt Synonym Gene Names: HsPUM; Pumilio-1

NCBI Description

This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (PubMed:21572425, PubMed:18328718, PubMed:21653694, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:20818387, PubMed:20860814, PubMed:22345517). Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle (PubMed:20818387). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed:26724866). Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery (PubMed:25768905). Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845). In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation (). Binds specifically to miRNA MIR199A precursor, with PUM2, regulates miRNA MIR199A expression at a postranscriptional level (PubMed:28431233).