Human Protocadherin 20 (PCDH20) ELISA Kit | PCDH20 elisa kit

Human Protocadherin 20 (PCDH20) ELISA Kit

Cat. Num. AAA2705361

• Gene Names: PCDH20; PCDH13
• Reactivity: Human
• Synonyms: Protocadherin 20 (PCDH20); Human Protocadherin 20 (PCDH20) ELISA Kit; PCDH13; Protocadherin-13; PCDH20 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protocadherin 20 (PCDH20).; No significant cross-reactivity or interference between Protocadherin 20 (PCDH20) and analogues was observed.
• Sequence Length: 951

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.126ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protocadherin 20 (PCDH20) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protocadherin 20 (PCDH20) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PCDH20 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protocadherin 20 (PCDH20). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protocadherin 20 (PCDH20). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protocadherin 20 (PCDH20), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protocadherin 20 (PCDH20) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 190194360
• NCBI GeneID: 64881
• NCBI Accession #: NP_073754.2
• NCBI GenBank Nucleotide #: NM_022843.3
• UniProt Accession #: Q8N6Y1; Q8NDN4; Q9NRT9; A8K1K9; B1AQU2
• Molecular Weight: 104,919 Da
• NCBI Official Full Name: protocadherin-20
• NCBI Official Synonym Full Names: protocadherin 20
• NCBI Official Symbol: PCDH20
• NCBI Official Synonym Symbols: PCDH13
• NCBI Protein Information: protocadherin-20
• UniProt Protein Name: Protocadherin-20
• Protein Family: Protocadherin
• UniProt Gene Name: PCDH20
• UniProt Synonym Gene Names: PCDH13

NCBI Description

This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. This gene encodes a protein which contains 6 extracellular cadherin domains, a transmembrane domain and a cytoplasmic tail differing from those of the classical cadherins. Although its specific function is undetermined, the cadherin-related neuronal receptor is thought to play a role in the establishment and function of specific cell-cell connections in the brain. [provided by RefSeq, Jul 2008]

Uniprot Description

PCDH20: Potential calcium-dependent cell-adhesion protein.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q21.2

Cellular Component: integral component of membrane; plasma membrane

Molecular Function: calcium ion binding; RNA binding

Biological Process: homophilic cell adhesion