Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein Z-dependent protease inhibitor (SERPINA10) Standard Curve.)
Human Protein Z-Dependent Protease Inhibitor (SERPINA10) ELISA Kit | SERPINA10 elisa kit
Human Protein Z-Dependent Protease Inhibitor (SERPINA10) ELISA Kit
Gene Names
SERPINA10; PZI; ZPI
Reactivity
Human
Synonyms
Protein Z-Dependent Protease Inhibitor (SERPINA10); Human Protein Z-Dependent Protease Inhibitor (SERPINA10) ELISA Kit; SERPINA10; PZI; ZPI; protein Z-dependent protease inhibitor; protein Z-dependent protease inhibitorprecursor; serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 10; SERPINA10 elisa kit
Reactivity
Human
Specificity
Human Protein Z-dependent protease inhibitor (SERPINA10) ELISA Kit has high sensitivity and excellent specificity for detection of Human SERPINA10. No significant cross-reactivity or interference between Human SERPINA10 and analogues was observed.
Sequence Length
444
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein Z-dependent protease inhibitor (SERPINA10) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein Z-dependent protease inhibitor (SERPINA10) Standard Curve.)
Related Product Information for SERPINA10 elisa kit
Description: This Human Protein Z-dependent protease inhibitor (SERPINA10) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINA10 in samples. An antibody specific for SERPINA10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINA10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Protein Z-dependent protease inhibitor is a protein circulating in the blood which inhibits factors Xa and XIa of the coagulation cascade. It is a member of the class of the serine protease inhibitors (serpins). Its name implies that it requires protein Z, another circulating protein, to function properly, but this only applies to its inhibition of factor X. It is about 72 kDa heavy and 444 amino acids large. It is produced by the liver.Water et al. found deficiency of ZPI in 4.4% of a cohort of patients with thrombophilia (a tendency to thrombosis).Han et al. first described ZPI in 1998. The same group further characterised it in 2000.
Background: Protein Z-dependent protease inhibitor is a protein circulating in the blood which inhibits factors Xa and XIa of the coagulation cascade. It is a member of the class of the serine protease inhibitors (serpins). Its name implies that it requires protein Z, another circulating protein, to function properly, but this only applies to its inhibition of factor X. It is about 72 kDa heavy and 444 amino acids large. It is produced by the liver.Water et al. found deficiency of ZPI in 4.4% of a cohort of patients with thrombophilia (a tendency to thrombosis).Han et al. first described ZPI in 1998. The same group further characterised it in 2000.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein Z-dependent protease inhibitor
NCBI Official Synonym Full Names
serpin family A member 10
NCBI Official Symbol
SERPINA10
NCBI Official Synonym Symbols
PZI; ZPI
NCBI Protein Information
protein Z-dependent protease inhibitor
UniProt Protein Name
Protein Z-dependent protease inhibitor
Protein Family
UniProt Gene Name
SERPINA10
UniProt Synonym Gene Names
ZPI; PZ-dependent protease inhibitor; PZI
UniProt Entry Name
ZPI_HUMAN
NCBI Description
The protein encoded by this gene belongs to the serpin family. It is predominantly expressed in the liver and secreted in plasma. It inhibits the activity of coagulation factors Xa and XIa in the presence of protein Z, calcium and phospholipid. Mutations in this gene are associated with venous thrombosis. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, May 2010]
Uniprot Description
SERPINA10: Inhibits activity of the coagulation protease factor Xa in the presence of PROZ, calcium and phospholipids. Belongs to the serpin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 14q32.13
Cellular Component: extracellular space
Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity
Biological Process: regulation of acrosome reaction; blood coagulation
Research Articles on SERPINA10
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Product Notes
The Human SERPINA10 serpina10 (Catalog #AAA9714371) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714371 ELISA Kit recognizes Human SERPINA10. It is sometimes possible for the material contained within the vial of "Protein Z-Dependent Protease Inhibitor (SERPINA10), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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