Human Protein VPRBP (VPRBP) ELISA Kit | VPRBP elisa kit

Human Protein VPRBP (VPRBP) ELISA Kit

Cat. Num. AAA286708

• Gene Names: DCAF1; RIP; VPRBP
• Reactivity: Human
• Synonyms: Protein VPRBP (VPRBP); Human Protein VPRBP (VPRBP) ELISA Kit; DCAF1; KIAA0800; MGC102804; DDB1 and CUL4 associated factor 1; HIV-1 Vpr binding protein; Vpr-binding protein; VPRBP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human VPRBP. No significant cross-reactivity or interference between Human VPRBP and analogues was observed.
• Sequence Length: 1506

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for VPRBP elisa kit

This assay employs a two-site sandwich ELISA to quantitate VPRBP in samples. An antibody specific for VPRBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VPRBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VPRBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VPRBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 284813529
• NCBI GeneID: 9730
• NCBI Accession #: NP_001165375.1
• NCBI GenBank Nucleotide #: NM_001171904.1
• UniProt Accession #: Q9Y4B6; Q2YD74; Q8TBD9; Q9HCA1; Q9UG37
• Molecular Weight: 118,235 Da
• NCBI Official Full Name: DDB1- and CUL4-associated factor 1 isoform 2
• NCBI Official Synonym Full Names: DDB1 and CUL4 associated factor 1
• NCBI Official Symbol: DCAF1
• NCBI Official Synonym Symbols: RIP; VPRBP
• NCBI Protein Information: DDB1- and CUL4-associated factor 1; protein VPRBP
• UniProt Protein Name: DDB1- and CUL4-associated factor 1
• Protein Family: Protein
• UniProt Gene Name: DCAF1
• UniProt Synonym Gene Names: VprBP

Uniprot Description

Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification. Probable substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2. Involved in the turnover of methylated proteins: recognizes and binds methylated proteins via its chromo domain, leading to ubiquitination of target proteins by the RBX1-DDB1-DCAF1/VPRBP complex (PubMed:23063525). The CUL4A-RBX1-DDB1-DCAF1/VPRBP complex is also involved in B-cell development: DCAF1 is recruited by RAG1 to ubiquitinate proteins, leading to limit error-prone repair during V(D)J recombination. Also part of the EDVP complex, an E3 ligase complex that mediates ubiquitination of proteins such as TERT, leading to TERT degradation and telomerase inhibition (PubMed:23362280). Also acts as an atypical serine/threonine-protein kinase that specifically mediates phosphorylation of 'Thr-120' of histone H2A (H2AT120ph) in a nucleosomal context, thereby repressing transcription. H2AT120ph is present in the regulatory region of many tumor suppresor genes, down-regulates their transcription and is present at high level in a number of tumors (PubMed:24140421). Involved in JNK-mediated apoptosis during cell competition process via its interaction with LLGL1 and LLGL2 (PubMed:20644714).