Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein LYRIC (MTDH) Standard Curve.)
Human Protein LYRIC (MTDH) ELISA Kit | MTDH elisa kit
Human Protein LYRIC (MTDH) ELISA Kit
Gene Names
MTDH; 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
Reactivity
Human
Synonyms
Protein LYRIC (MTDH); Human Protein LYRIC (MTDH) ELISA Kit; MTDH; 3D3; AEG-1; AEG1; LYRIC; LYRIC/3D3; astrocyte elevated gene-1; metastasis adhesion protein; MTDH elisa kit
Reactivity
Human
Specificity
Human Protein LYRIC (MTDH) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTDH. No significant cross-reactivity or interference between Human MTDH and analogues was observed.
Sequence Length
582
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein LYRIC (MTDH) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein LYRIC (MTDH) Standard Curve.)
Related Product Information for MTDH elisa kit
Description: This Human Protein LYRIC (MTDH) ELISA Kit employs a two-site sandwich ELISA to quantitate MTDH in samples. An antibody specific for MTDH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MTDH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTDH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTDH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MTDH (Metadherin) is a Protein Coding gene. Diseases associated with MTDH include Nervous System Disease. Among its related pathways are Adhesion and Cytoskeleton remodeling Regulation of actin cytoskeleton by Rho GTPases. GO annotations related to this gene include poly(A) RNA binding and double-stranded RNA binding. Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
Background: MTDH (Metadherin) is a Protein Coding gene. Diseases associated with MTDH include Nervous System Disease. Among its related pathways are Adhesion and Cytoskeleton remodeling Regulation of actin cytoskeleton by Rho GTPases. GO annotations related to this gene include poly(A) RNA binding and double-stranded RNA binding. Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein LYRIC isoform 1
NCBI Official Synonym Full Names
metadherin
NCBI Official Symbol
MTDH
NCBI Official Synonym Symbols
3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
NCBI Protein Information
protein LYRIC
UniProt Protein Name
Protein LYRIC
Protein Family
UniProt Gene Name
MTDH
UniProt Synonym Gene Names
AEG1; LYRIC; AEG-1
UniProt Entry Name
LYRIC_HUMAN
Uniprot Description
Lyric: a protein of unknown function.
Protein type: Cell adhesion; Nucleolus; Membrane protein, integral
Chromosomal Location of Human Ortholog: 8q22.1
Cellular Component: apical plasma membrane; cytoplasm; endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; intercellular canaliculus; nuclear body; nuclear membrane; nucleolus; nucleus; perinuclear region of cytoplasm; tight junction
Molecular Function: double-stranded RNA binding; NF-kappaB binding; protein binding; transcription coactivator activity
Biological Process: activation of NF-kappaB transcription factor; lipopolysaccharide-mediated signaling pathway; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; positive regulation of angiogenesis; positive regulation of autophagy; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of protein kinase B signaling cascade
Research Articles on MTDH
Similar Products
Product Notes
The Human MTDH mtdh (Catalog #AAA9715168) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715168 ELISA Kit recognizes Human MTDH. It is sometimes possible for the material contained within the vial of "Protein LYRIC (MTDH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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