Human Protein Arginine Methyltransferase 1 (PRMT1) ELISA Kit | PRMT1 elisa kit

Human Protein Arginine Methyltransferase 1 (PRMT1) ELISA Kit

Cat. Num. AAA2098053

• Gene Names: Prmt1; Mrmt1; Hrmt1l2; AW214366; 6720434D09Rik
• Reactivity: Human
• Synonyms: Protein Arginine Methyltransferase 1 (PRMT1); Human Protein Arginine Methyltransferase 1 (PRMT1) ELISA Kit; ANM1; HCP1; HRMT1L2; IR1B4; Histone-arginine N-methyltransferase PRMT1; Interferon receptor 1-bound protein 4; PRMT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Arginine Methyltransferase 1 (PRMT1).; No significant cross-reactivity or interference between Protein Arginine Methyltransferase 1 (PRMT1) and analogues was observed.
• Sequence Length: 353

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Arginine Methyltransferase 1 (PRMT1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Arginine Methyltransferase 1 (PRMT1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRMT1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Arginine Methyltransferase 1 (PRMT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Arginine Methyltransferase 1 (PRMT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Arginine Methyltransferase 1 (PRMT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Arginine Methyltransferase 1 (PRMT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PRMT1 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 357197158
• NCBI GeneID: 15469
• NCBI Accession #: NP_001239405.1
• NCBI GenBank Nucleotide #: NM_001252476.1
• UniProt Accession #: Q9JIF0; Q99LS4
• Molecular Weight: 39,576 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 1 isoform 2
• NCBI Official Synonym Full Names: protein arginine N-methyltransferase 1
• NCBI Official Symbol: Prmt1
• NCBI Official Synonym Symbols: Mrmt1; Hrmt1l2; AW214366; 6720434D09Rik
• NCBI Protein Information: protein arginine N-methyltransferase 1
• UniProt Protein Name: Protein arginine N-methyltransferase 1
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: Prmt1
• UniProt Synonym Gene Names: Hrmt1l2; Mrmt1

Uniprot Description

Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4 and SERBP1. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity. Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (). Promotes colorectal cancer cell malignancy ().