Human Protein amnionless (AMN) ELISA Kit | AMN elisa kit

Human Protein amnionless (AMN) ELISA Kit

Cat. Num. AAA2800251

• Gene Names: AMN; PRO1028; amnionless
• Reactivity: Human
• Synonyms: Protein amnionless (AMN); Human Protein amnionless (AMN) ELISA Kit; PRO1028; amnionless protein; visceral endoderm-specific type 1 transmembrane protein; AMN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human AMN. No significant cross-reactivity or interference between Human AMN and analogues was observed.
• Sequence Length: 453

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for AMN elisa kit

This assay employs a two-site sandwich ELISA to quantitate AMN in samples. An antibody specific for AMN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AMN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMN bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 110611172
• NCBI GeneID: 81693
• NCBI Accession #: NP_112205.2
• NCBI GenBank Nucleotide #: NM_030943.3
• UniProt Accession #: Q9BXJ7; Q6UX83
• Molecular Weight: 47,754 Da
• NCBI Official Full Name: protein amnionless
• NCBI Official Synonym Full Names: amnion associated transmembrane protein
• NCBI Official Symbol: AMN
• NCBI Official Synonym Symbols: PRO1028; amnionless
• NCBI Protein Information: protein amnionless
• UniProt Protein Name: Protein amnionless
• Protein Family: Protein amnionless
• UniProt Gene Name: AMN

NCBI Description

The protein encoded by this gene is a type I transmembrane protein. It is thought to modulate bone morphogenetic protein (BMP) receptor function by serving as an accessory or coreceptor, and thus facilitates or hinders BMP binding. It is known that the mouse AMN gene is expressed in the extraembryonic visceral endoderm layer during gastrulation, but it is found to be mutated in amnionless mouse. The encoded protein has sequence similarity to short gastrulation (Sog) and procollagen IIA proteins in Drosophila. [provided by RefSeq, Jul 2008]

Uniprot Description

Necessary for efficient absorption of vitamin B12 (PubMed:12590260, PubMed:14576052). Required for normal CUBN-mediated protein transport in the kidney. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm ().