Human Proteasome Subunit Alpha Type 5 (PSMa5) ELISA Kit | PSMa5 elisa kit

Human Proteasome Subunit Alpha Type 5 (PSMa5) ELISA Kit

Cat. Num. AAA455022

• Gene Names: PSMA5; PSC5; ZETA
• Reactivity: Human
• Synonyms: Proteasome Subunit Alpha Type 5 (PSMa5); Human Proteasome Subunit Alpha Type 5 (PSMa5) ELISA Kit; PSC5; Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome zeta chain; PSMa5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PSMa5. No significant cross-reactivity or interference between PSMa5 and analogues was observed.
• Sequence Length: 183

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.114ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PSMa5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PSMa5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSMa5 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PSMa5 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PSMa5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PSMa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PSMa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PSMa5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 315138981
• NCBI GeneID: 5686
• NCBI Accession #: NP_001186701.1
• NCBI GenBank Nucleotide #: NM_001199772.1
• UniProt Accession #: P28066; Q3T1C1; Q6IBF7; B2R8F6; B4E2V4
• Molecular Weight: 20,035 Da
• NCBI Official Full Name: proteasome subunit alpha type-5 isoform 2
• NCBI Official Synonym Full Names: proteasome subunit alpha 5
• NCBI Official Symbol: PSMA5
• NCBI Official Synonym Symbols: PSC5; ZETA
• NCBI Protein Information: proteasome subunit alpha type-5
• UniProt Protein Name: Proteasome subunit alpha type-5
• UniProt Gene Name: PSMA5

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.