Human Proteasome 26S Subunit, Non ATPase 10 (PSMD10) ELISA Kit | PSMD10 elisa kit

Human Proteasome 26S Subunit, Non ATPase 10 (PSMD10) ELISA Kit

Cat. Num. AAA451629

• Gene Names: PSMD10; p28; p28(GANK); dJ889N15.2
• Reactivity: Human
• Synonyms: Proteasome 26S Subunit; Non ATPase 10 (PSMD10); Human Proteasome 26S Subunit; Non ATPase 10 (PSMD10) ELISA Kit; p28; Gankyrin; 26S proteasome non-ATPase regulatory subunit 10; 26S proteasome regulatory subunit p28; PSMD10 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PSMD10. No significant cross-reactivity or interference between PSMD10 and analogues was observed.
• Sequence Length: 226

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.059ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PSMD10 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PSMD10 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSMD10 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PSMD10 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PSMD10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PSMD10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PSMD10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PSMD10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506217
• NCBI GeneID: 5716
• NCBI Accession #: NP_002805.1
• NCBI GenBank Nucleotide #: NM_002814.3
• UniProt Accession #: O75832; Q5U0B2; Q8IZK9
• Molecular Weight: 16,137 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 10 isoform 1
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 10
• NCBI Official Symbol: PSMD10
• NCBI Official Synonym Symbols: p28; p28(GANK); dJ889N15.2
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 10
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 10
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD10

NCBI Description

This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.[provided by RefSeq, Mar 2011]

Uniprot Description

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pahway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.