Rabbit anti-Human NOS2 / iNOS Polyclonal Antibody | anti-NOS2 / iNOS antibody

Anti-NOS2 / iNOS Antibody IHC-plus

Cat. Num. AAA249835

• Gene Names: NOS2; NOS; INOS; NOS2A; HEP-NOS
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Affinity purified
• Synonyms: NOS2 / iNOS; Polyclonal Antibody; Anti-NOS2 / iNOS Antibody IHC-plus; Rabbit Polyclonal (IgG) to Human NOS2 / iNOS; Human NOS2 / iNOS; Hepatocyte NOS; HEP-NOS; Inducible NOS; NOS; type II; NOS type II; Inducible NO synthase; NOS2A; INOS; anti-NOS2 / iNOS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human NOS2 / iNOS
• Purity/Purification: Affinity purified
• Form/Format: PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1153

• Applicable Applications for anti-NOS2 / iNOS antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P (2.5 ug/ml); WB (1:500 - 1:1000)
• Immunogen: NOS2 / iNOS antibody was raised against a synthetic peptide of human NOS2.
• Immunogen Description: A synthetic peptide of human NOS2.
• Immunogen Type: Synthetic peptide
• Target Species: Human
• Preparation and Storage: Long term: -80 degree C; Short term: -20 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry - Paraffin (IHC)

(Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry - Paraffin (IHC)

(Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of CEM cell lysate using NOS2 antibody.)

Related Product Information for anti-NOS2 / iNOS antibody

NOS2 Antibody, Hepatocyte NOS Antibody, HEP-NOS Antibody, Inducible NOS Antibody, NOS, type II Antibody, NOS type II Antibody, Inducible NO synthase Antibody, NOS2A Antibody, INOS Antibody, NOS Antibody Description: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

NCBI and Uniprot Product Information

• NCBI GI #: 24041029
• NCBI GeneID: 4843
• NCBI Accession #: NP_000616.3
• NCBI GenBank Nucleotide #: NM_000625.4
• UniProt Accession #: P35228; O60757; O94994; Q16263; Q16692; Q4TTS5; Q9UD42; A1L3U5; B7ZLY2
• Molecular Weight: 126,749 Da
• NCBI Official Full Name: nitric oxide synthase, inducible
• NCBI Official Synonym Full Names: nitric oxide synthase 2
• NCBI Official Symbol: NOS2
• NCBI Official Synonym Symbols: NOS; INOS; NOS2A; HEP-NOS
• NCBI Protein Information: nitric oxide synthase, inducible
• UniProt Protein Name: Nitric oxide synthase, inducible
• UniProt Gene Name: NOS2
• UniProt Synonym Gene Names: NOS2A; HEP-NOS; Inducible NOS; iNOS
• UniProt Entry Name: NOS2_HUMAN

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cortical cytoskeleton; cytoplasm; cytosol; intracellular; nucleus; perinuclear region of cytoplasm; peroxisome

Molecular Function: calmodulin binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; nitric-oxide synthase activity; protein binding; protein homodimerization activity; receptor binding

Biological Process: arginine catabolic process; blood coagulation; cell redox homeostasis; circadian rhythm; defense response to bacterium; defense response to Gram-negative bacterium; inflammatory response; innate immune response in mucosa; negative regulation of blood pressure; negative regulation of protein catabolic process; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; peptidyl-cysteine S-nitrosylation; positive regulation of guanylate cyclase activity; positive regulation of killing of cells of another organism; positive regulation of leukocyte mediated cytotoxicity; positive regulation of vasodilation; prostaglandin secretion; regulation of cell proliferation; regulation of cellular respiration; regulation of insulin secretion; response to bacterium; response to hypoxia; superoxide metabolic process

Disease: Hypertension, Essential; Malaria, Susceptibility To