Typical Testing Data/Standard Curve (for reference only)

Human Prolactin ELISA Kit

Reactivity

Human

Synonyms

Prolactin; Human Prolactin ELISA Kit; Prolactin elisa kit

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Cat. Num. AAA9141999 For Research Use Only!

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of Prolactin. No significant cross-reactivity or interference between Prolactin and analogues was observed.

Samples

Cell culture supernates, serum and plasma

Assay Type

Quantitative Sandwich

Sensitivity

< 0.124ng/mL

Intra-assay Precision

Three samples of known concentration were tested 20 times on one plate to evaluate the Intra-plate precision.

Inter-assay Precision

Three samples of known concentration were tested 20 times separate assays to evaluate the Inter-plate precision.Assay were using two lots of components.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for Prolactin elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Prolactin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Prolactin present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for Prolactin is added to the wells and binds to the combination of capture antibody Prolactin in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps, a substrate is added. A colored product TMB is formed in proportion to the amount of Prolactin present in the sample. The reaction is terminated by addition of acid and absorbance is measured. A standard curve is prepared from seven Prolactin standard dilutions and Prolactin sample concentration determined.

Background/Introduction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Prolactin in cell culture supernates, serum and plasma.

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Product Notes

The Human Prolactin (Catalog #AAA9141999) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9141999 ELISA Kit recognizes Human Prolactin. It is sometimes possible for the material contained within the vial of "Prolactin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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Human Prolactin ELISA Kit