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Human Probable ATP-dependent RNA helicase DDX31 ELISA Kit | DDX31 elisa kit

Human Probable ATP-dependent RNA helicase DDX31 (DDX31) ELISA Kit

Gene Names
DDX31; PPP1R25
Reactivity
Human
Synonyms
Probable ATP-dependent RNA helicase DDX31; Human Probable ATP-dependent RNA helicase DDX31 (DDX31) ELISA Kit; DDX31 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human DDX31. No significant cross-reactivity or interference between Human DDX31 and analogues was observed.
Sequence Length
851
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for DDX31 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DDX31 in samples. An antibody specific for DDX31 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDDX31 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DDX31 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DDX31 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
64,137 Da
NCBI Official Full Name
probable ATP-dependent RNA helicase DDX31 isoform 3
NCBI Official Synonym Full Names
DEAD-box helicase 31
NCBI Official Symbol
DDX31
NCBI Official Synonym Symbols
PPP1R25
NCBI Protein Information
probable ATP-dependent RNA helicase DDX31
UniProt Protein Name
Probable ATP-dependent RNA helicase DDX31
UniProt Gene Name
DDX31

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2016]

Uniprot Description

DDX31: Probable ATP-dependent RNA helicase. Belongs to the DEAD box helicase family. DDX31/DBP7 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.13; Hydrolase; Nucleolus

Chromosomal Location of Human Ortholog: 9q34.13

Cellular Component: cytoplasm; Golgi apparatus; intracellular membrane-bound organelle; nucleolus

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; protein binding; RNA binding

Biological Process: ribosome biogenesis; RNA secondary structure unwinding

Research Articles on DDX31

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Product Notes

The Human DDX31 ddx31 (Catalog #AAA281618) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281618 ELISA Kit recognizes Human DDX31. It is sometimes possible for the material contained within the vial of "Probable ATP-dependent RNA helicase DDX31, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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