Human Probable ATP-dependent RNA helicase DDX28 ELISA Kit | DDX28 elisa kit

Human Probable ATP-dependent RNA helicase DDX28 (DDX28) ELISA Kit

Cat. Num. AAA280402

• Gene Names: DDX28; MDDX28
• Reactivity: Human
• Synonyms: Probable ATP-dependent RNA helicase DDX28; Human Probable ATP-dependent RNA helicase DDX28 (DDX28) ELISA Kit; DDX28 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human DDX28. No significant cross-reactivity or interference between Human DDX28 and analogues was observed.
• Sequence Length: 540

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for DDX28 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DDX28 in samples. An antibody specific for DDX28 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDDX28 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DDX28 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DDX28 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 256773275
• NCBI GeneID: 55794
• NCBI Accession #: NP_060850.2
• NCBI GenBank Nucleotide #: NM_018380.3
• UniProt Accession #: Q9NUL7
• Molecular Weight: 59,581 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX28
• NCBI Official Synonym Full Names: DEAD-box helicase 28
• NCBI Official Symbol: DDX28
• NCBI Official Synonym Symbols: MDDX28
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX28
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX28
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX28
• UniProt Synonym Gene Names: MDDX28

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus. [provided by RefSeq, Jul 2008]

Uniprot Description

DDX28: May be involved in RNA processing or transport. Has RNA and Mg(2+)-dependent ATPase activity. Belongs to the DEAD box helicase family.

Protein type: EC 3.6.4.13; Hydrolase; RNA-binding

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cytosol; mitochondrion; nucleolus; nucleus

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; RNA binding; rRNA binding

Biological Process: RNA secondary structure unwinding