Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Probable arylformamidase (AFMID) Standard Curve.)
Human Probable Arylformamidase (AFMID) ELISA Kit | AFMID elisa kit
Human Probable Arylformamidase (AFMID) ELISA Kit
Gene Names
AFMID; KF; FKF; KFA
Reactivity
Human
Synonyms
Probable Arylformamidase (AFMID); Human Probable Arylformamidase (AFMID) ELISA Kit; AFMID; DKFZp686F03259; KF; MGC167063; AFMID elisa kit
Reactivity
Human
Specificity
Human Probable arylformamidase (AFMID) ELISA Kit has high sensitivity and excellent specificity for detection of Human AFMID. No significant cross-reactivity or interference between Human AFMID and analogues was observed.
Sequence Length
303
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Probable arylformamidase (AFMID) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Probable arylformamidase (AFMID) Standard Curve.)
Related Product Information for AFMID elisa kit
Description: This Human Probable arylformamidase (AFMID) ELISA Kit employs a two-site sandwich ELISA to quantitate AFMID in samples. An antibody specific for AFMID has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AFMID present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFMID is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFMID bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Arylformamidase (AFMID) is the second enzyme of the kynurenine pathway metabolizing tryptophan to nicotinic acid and nicotinamide adenine dinucleotide cofactors. Inhibition of AFMID by organophosphorus insecticides in developing chicken embryos is correlated with lowered NAD levels and severe teratogenesis. The cDNA sequence previously identified for mouse liver AFMID was cloned and expressed in Escherichia coli. Residues identified as potential catalytic triad members through sequence motif and homology modeling were mutated to alanine to probe their contributions to enzyme activity. The wild-type and mutant AFMIDs were expressed as amino terminal 6 x His-tagged recombinant proteins to facilitate purification. Three chromatography steps isolated highly purified proteins for enzyme activity comparisons.
Background: Arylformamidase (AFMID) is the second enzyme of the kynurenine pathway metabolizing tryptophan to nicotinic acid and nicotinamide adenine dinucleotide cofactors. Inhibition of AFMID by organophosphorus insecticides in developing chicken embryos is correlated with lowered NAD levels and severe teratogenesis. The cDNA sequence previously identified for mouse liver AFMID was cloned and expressed in Escherichia coli. Residues identified as potential catalytic triad members through sequence motif and homology modeling were mutated to alanine to probe their contributions to enzyme activity. The wild-type and mutant AFMIDs were expressed as amino terminal 6 x His-tagged recombinant proteins to facilitate purification. Three chromatography steps isolated highly purified proteins for enzyme activity comparisons.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
kynurenine formamidase isoform 2
NCBI Official Synonym Full Names
arylformamidase
NCBI Official Symbol
AFMID
NCBI Official Synonym Symbols
KF; FKF; KFA
NCBI Protein Information
kynurenine formamidase
UniProt Protein Name
Kynurenine formamidase
UniProt Gene Name
AFMID
UniProt Synonym Gene Names
KFA; KFase; FKF
Uniprot Description
AFMID: Catalyzes the hydrolysis of N-formyl-L-kynurenine to L- kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites. Belongs to the kynurenine formamidase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - tryptophan; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 3.5.1.9; Hydrolase
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: cytoplasm; cytosol; nucleus
Molecular Function: arylformamidase activity; hydrolase activity
Biological Process: tryptophan catabolic process to kynurenine
Research Articles on AFMID
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Product Notes
The Human AFMID afmid (Catalog #AAA9714621) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714621 ELISA Kit recognizes Human AFMID. It is sometimes possible for the material contained within the vial of "Probable Arylformamidase (AFMID), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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