Human Pregnenolone ELISA Kit
Pregnenolone ELISA
Reactivity
Human
Synonyms
Pregnenolone; Pregnenolone ELISA; Pregnenolone elisa kit
Reactivity
Human
Samples
Human serum
Assay Type
Competitive
Detection Range
0 / 0.1 - 25.6 ng/mL
Sensitivity
0.05 ng/mL
Standards
6 calibrators, ready to use.
Sample Volume
50 uL
Design
Enzyme immunoassay technique.
Controls
2 controls, ready to use.
Assay Description
1 hour (RT) + 15 min. (RT) = 1 hour, 15 min. total clock time.
Preparation and Storage
2 - 8 degree C
Related Product Information for Pregnenolone elisa kit
Intended Use For the direct quantitative determination of Pregnenolone by enzyme immunoassay in human serum. For Research Use Only.
Principle of the test The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards,control and samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. Theabsorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of pregnenolone in the sample. A set of standards is used to plot a standard curve from which the amount of pregnenolone in patient samples and controls can be directly read.
Principle of the test The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards,control and samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. Theabsorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of pregnenolone in the sample. A set of standards is used to plot a standard curve from which the amount of pregnenolone in patient samples and controls can be directly read.
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Product Notes
The Human Pregnenolone (Catalog #AAA494159) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494159 ELISA Kit recognizes Human Pregnenolone. It is sometimes possible for the material contained within the vial of "Pregnenolone, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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