Human Pre-mRNA-splicing factor CWC22 homolog ELISA Kit | CWC22 elisa kit
Human Pre-mRNA-splicing factor CWC22 homolog (CWC22) ELISA Kit
Reactivity
Human
Synonyms
Pre-mRNA-splicing factor CWC22 homolog; Human Pre-mRNA-splicing factor CWC22 homolog (CWC22) ELISA Kit; CWC22 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human CWC22. No significant cross-reactivity or interference between Human CWC22 and analogues was observed.
Sequence Length
908
Samples
serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.31 ng/mL - 20 ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for CWC22 elisa kit
Intended Uses: For the quantitative detection of Human Pre-mRNA-splicing factor CWC22 homolog (CWC22) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CWC22 in Human serum, plasma. An antibody specific for CWC22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CWC22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CWC22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CWC22 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CWC22 in Human serum, plasma. An antibody specific for CWC22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CWC22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CWC22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CWC22 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
108,675 Da
NCBI Official Full Name
Pre-mRNA-splicing factor CWC22 homolog
UniProt Protein Name
Pre-mRNA-splicing factor CWC22 homolog
Protein Family
UniProt Gene Name
CWC22
UniProt Synonym Gene Names
NCM
Uniprot Description
Required for pre-mRNA splicing and for exon-junction complex (EJC) assembly. Hinders EIF4A3 from non-specifically binding RNA and escorts it to the splicing machinery to promote EJC assembly on mature mRNAs. Through its role in EJC assembly, required for nonsense-mediated mRNA decay ().
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Product Notes
The Human CWC22 cwc22 (Catalog #AAA280632) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280632 ELISA Kit recognizes Human CWC22. It is sometimes possible for the material contained within the vial of "Pre-mRNA-splicing factor CWC22 homolog, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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