Human Poly (U)-binding-splicing factor PUF60 ELISA Kit | PUF60 elisa kit

Human Poly (U)-binding-splicing factor PUF60 (PUF60) ELISA Kit

Cat. Num. AAA280488

• Gene Names: PUF60; FIR; VRJS; RoBPI; SIAHBP1
• Reactivity: Human
• Synonyms: Poly (U)-binding-splicing factor PUF60; Human Poly (U)-binding-splicing factor PUF60 (PUF60) ELISA Kit; PUF60 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PUF60. No significant cross-reactivity or interference between Human PUF60 and analogues was observed.
• Sequence Length: 499

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PUF60 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PUF60 in samples. An antibody specific for PUF60 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPUF60 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PUF60 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PUF60 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 209862867
• NCBI GeneID: 22827
• NCBI Accession #: NP_001129505.1
• NCBI GenBank Nucleotide #: NM_001136033.2
• UniProt Accession #: Q9UHX1; Q969E7; Q96D94; Q96H63; Q99628; Q9NZA0; Q9UJY7; A8K8K8
• Molecular Weight: 55,399 Da
• NCBI Official Full Name: poly(U)-binding-splicing factor PUF60 isoform c
• NCBI Official Synonym Full Names: poly(U) binding splicing factor 60
• NCBI Official Symbol: PUF60
• NCBI Official Synonym Symbols: FIR; VRJS; RoBPI; SIAHBP1
• NCBI Protein Information: poly(U)-binding-splicing factor PUF60
• UniProt Protein Name: Poly(U)-binding-splicing factor PUF60
• Protein Family: Poly(U)-binding-splicing factor
• UniProt Gene Name: PUF60
• UniProt Synonym Gene Names: FIR; ROBPI; SIAHBP1; FBP-interacting repressor; RoBP1; Siah-BP1

NCBI Description

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Uniprot Description

FIR: fuse-binding protein-interacting repressor. Interacts with (and may activate) Ro RNPs. Forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. Two alternatively spliced isoforms have been described.

Protein type: RNA splicing; RNA-binding; Spliceosome; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cell junction; cyclin-dependent protein kinase activating kinase holoenzyme complex; intracellular ribonucleoprotein complex; nucleoplasm

Molecular Function: cadherin binding; DNA binding; identical protein binding; protein binding; RNA binding

Biological Process: apoptosis; mRNA splicing, via spliceosome; regulation of transcription, DNA-templated; transcription, DNA-dependent

Disease: Verheij Syndrome