Typical Testing Data/Standard Curve (for reference only)
Human Pleckstrin (PLEK) ELISA Kit | PLEK elisa kit
Human Pleckstrin (PLEK) ELISA Kit
Gene Names
PLEK; P47
Reactivity
Human
Synonyms
Pleckstrin (PLEK); Human Pleckstrin (PLEK) ELISA Kit; P47; Platelet 47 kDa protein; PLEK elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Pleckstrin (PLEK).
No significant cross-reactivity or interference between Pleckstrin (PLEK) and analogues was observed.
No significant cross-reactivity or interference between Pleckstrin (PLEK) and analogues was observed.
Sequence Length
350
Assay Type
Double-antibody Sandwich
Sample
Tissue Homogenates, Cell Lysates and Other Biological Fluids
Dectection Range
0.156-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.059ng/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pleckstrin (PLEK) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pleckstrin (PLEK) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pleckstrin (PLEK) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PLEK elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pleckstrin (PLEK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pleckstrin (PLEK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pleckstrin (PLEK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pleckstrin (PLEK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for PLEK elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pleckstrin
NCBI Official Synonym Full Names
pleckstrin
NCBI Official Symbol
PLEK
NCBI Official Synonym Symbols
P47
NCBI Protein Information
pleckstrin
UniProt Protein Name
Pleckstrin
Protein Family
UniProt Gene Name
PLEK
UniProt Synonym Gene Names
P47; p47
UniProt Entry Name
PLEK_HUMAN
Uniprot Description
pleckstrin: Major protein kinase C substrate of platelets.
Protein type: Lipid-binding
Chromosomal Location of Human Ortholog: 2p13.3
Cellular Component: membrane; cytoplasm; extracellular region; cytosol
Molecular Function: protein binding; protein homodimerization activity; protein kinase C binding; phosphatidylinositol-3,4-bisphosphate binding
Biological Process: negative regulation of calcium-mediated signaling; integrin-mediated signaling pathway; platelet activation; phospholipase C inhibition; positive regulation of actin filament depolymerization; negative regulation of G-protein coupled receptor protein signaling pathway; positive regulation of integrin activation; platelet degranulation; cell projection organization and biogenesis; actin cytoskeleton reorganization; ruffle organization and biogenesis; positive regulation of actin filament bundle formation; hemopoietic progenitor cell differentiation; phosphatidylinositol metabolic process; cortical actin cytoskeleton organization and biogenesis; blood coagulation; vesicle docking during exocytosis
Research Articles on PLEK
Similar Products
Product Notes
The Human PLEK plek (Catalog #AAA2122036) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2122036 ELISA Kit recognizes Human PLEK. It is sometimes possible for the material contained within the vial of "Pleckstrin (PLEK), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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