Western Blot (WB)
(Western Blot analysis of HL-60 cells using PLEK Monoclonal Antibody at dilution of 1:5000)
Mouse anti-Human PLEK Monoclonal Antibody | anti-PLEK antibody
PLEK Monoclonal Antibody
Gene Names
PLEK; P47
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Protein G purification
Synonyms
PLEK; Monoclonal Antibody; PLEK Monoclonal Antibody; FLJ27168; OTTHUMP00000159962; p47; Platelet 47 kDa protein; Platelet and leukocyte C kinase substrate; Platelet P47; Platelet p47 protein; Pleckstrin; anti-PLEK antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1D5C2
Purity/Purification
Protein G purification
Concentration
1mg/mL (varies by lot)
Sequence Length
350
Applicable Applications for anti-PLEK antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:2000-1:20000
IHC: 1:50-1:500
IHC: 1:50-1:500
Immunogen
Fusion protein of PLEK
Buffer
PBS with 0.02% sodium azide, 50% glycerol, PH7.3
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of HL-60 cells using PLEK Monoclonal Antibody at dilution of 1:5000)
Western Blot (WB)
(Western Blot analysis of HL-60 cells using PLEK Monoclonal Antibody at dilution of 1:5000)
Related Product Information for anti-PLEK antibody
PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)
Product Categories/Family for anti-PLEK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 40kDa
Observed: 40-47kDa
Observed: 40-47kDa
NCBI Official Full Name
pleckstrin
NCBI Official Synonym Full Names
pleckstrin
NCBI Official Symbol
PLEK
NCBI Official Synonym Symbols
P47
NCBI Protein Information
pleckstrin
UniProt Protein Name
Pleckstrin
Protein Family
UniProt Gene Name
PLEK
UniProt Synonym Gene Names
P47; p47
Uniprot Description
Major protein kinase C substrate of platelets.
Research Articles on PLEK
Similar Products
Product Notes
The PLEK plek (Catalog #AAA2541363) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PLEK Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLEK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:2000-1:20000 IHC: 1:50-1:500. Researchers should empirically determine the suitability of the PLEK plek for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLEK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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