Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphatase and tension homolog deleted on chromosome ten (PTEN/MMAC1) Standard Curve.)
Human Phosphatase And Tension Homolog Deleted On Chromosome Ten (PTEN/MMAC1) ELISA Kit | PTEN/MMAC1 elisa kit
Human Phosphatase And Tension Homolog Deleted On Chromosome Ten (PTEN/MMAC1) ELISA Kit
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
Reactivity
Human
Synonyms
Phosphatase And Tension Homolog Deleted On Chromosome Ten (PTEN/MMAC1); Human Phosphatase And Tension Homolog Deleted On Chromosome Ten (PTEN/MMAC1) ELISA Kit; PTEN; 10q23del; BZS; MGC11227; MHAM; MMAC1; PTEN1; TEP1; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; mutated in multiple advanced cancers 1; PTEN/MMAC1 elisa kit
Reactivity
Human
Specificity
Human Phosphatase and tension homolog deleted on chromosome ten (PTEN/MMAC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTEN. No significant cross-reactivity or interference between Human PTEN and analogues was observed.
Sequence Length
403
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphatase and tension homolog deleted on chromosome ten (PTEN/MMAC1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphatase and tension homolog deleted on chromosome ten (PTEN/MMAC1) Standard Curve.)
Related Product Information for PTEN/MMAC1 elisa kit
Description: This Human Phosphatase and tension homolog deleted on chromosome ten (PTEN/MMAC1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTEN in samples. An antibody specific for PTEN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTEN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTEN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTEN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PTEN acts as a tumor suppressor gene through the action of its phosphatase protein product. This phosphatase is involved in the regulation of the cell cycle, preventing cells from growing and dividing too rapidly.This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating Akt/PKB signaling pathway.
Background: PTEN acts as a tumor suppressor gene through the action of its phosphatase protein product. This phosphatase is involved in the regulation of the cell cycle, preventing cells from growing and dividing too rapidly.This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating Akt/PKB signaling pathway.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN isoform PTEN
NCBI Official Synonym Full Names
phosphatase and tensin homolog
NCBI Official Symbol
PTEN
NCBI Official Synonym Symbols
BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
NCBI Protein Information
phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
UniProt Protein Name
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
UniProt Gene Name
PTEN
UniProt Synonym Gene Names
MMAC1; TEP1
UniProt Entry Name
PTEN_HUMAN
NCBI Description
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2015]
Research Articles on PTEN/MMAC1
Similar Products
Product Notes
The Human PTEN/MMAC1 pten (Catalog #AAA9714739) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714739 ELISA Kit recognizes Human PTEN/MMAC1. It is sometimes possible for the material contained within the vial of "Phosphatase And Tension Homolog Deleted On Chromosome Ten (PTEN/MMAC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
