Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphatase and actin regulator 1 (PHACTR1) Standard Curve.)
Human Phosphatase And Actin Regulator 1 (PHACTR1) ELISA Kit | PHACTR1 elisa kit
Human Phosphatase And Actin Regulator 1 (PHACTR1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphatase and actin regulator 1 (PHACTR1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphatase and actin regulator 1 (PHACTR1) Standard Curve.)
Background: The PHACTR proteins share highest similarity in sequences surrounding the N- and C-terminal RPEL repeats and in the C-terminal actin- and PP1-binding domains. In situ hybridization of rat brain showed enrichment of Phactr1 in cerebral cortex, hippocampus, olfactory tubercle, nucleus accumbens, caudate-putamen, and piriform cortex. Endogenous PP1 coprecipitated with ectopically expressed rat Phactr1 in human embryonic kidney cells. Phactr1 also coprecipitated with actin in rat brain lysates. Yeast 2-hybrid analysis of a series of Phactr1 C-terminal truncation mutants indicated that PP1 bound to Phactr1 near the C terminus, while actin bound in a region containing RPEL repeats 2 and 3.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the phosphatase and actin regulator family of proteins. This family member can bind actin and regulate the reorganization of the actin cytoskeleton. It plays a role in tubule formation and in endothelial cell survival. Polymorphisms in this gene are associated with susceptibility to myocardial infarction, coronary artery disease and cervical artery dissection. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2016]
Uniprot Description
PHACTR1: Belongs to the phosphatase and actin regulator family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 6p24.1
Cellular Component: synapse; cell junction; cytosol; nucleus
Molecular Function: protein phosphatase type 1 regulator activity; protein phosphatase inhibitor activity; actin binding; protein phosphatase 1 binding
Biological Process: actomyosin structure organization and biogenesis; negative regulation of catalytic activity; actin cytoskeleton reorganization; stress fiber formation; cell motility involved in cell locomotion
Research Articles on PHACTR1
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Product Notes
The Human PHACTR1 phactr1 (Catalog #AAA9714888) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714888 ELISA Kit recognizes Human PHACTR1. It is sometimes possible for the material contained within the vial of "Phosphatase And Actin Regulator 1 (PHACTR1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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