Human Peroxisome Proliferator Activated Receptor Gamma (PPAR-gamma) ELISA Kit | PPAR-gamma elisa kit

Human Peroxisome Proliferator Activated Receptor Gamma (PPAR-gamma) ELISA Kit

Cat. Num. AAA263089

• Reactivity: Human
• Synonyms: Peroxisome Proliferator Activated Receptor Gamma (PPAR-gamma); Human Peroxisome Proliferator Activated Receptor Gamma (PPAR-gamma) ELISA Kit; Human Peroxisome Proliferator Activated Receptor Gamma (PPAR-g) ELISA Kit; PPAR-gamma elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 477

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 50 ng/ml-0.78ng/ml
• Sensitivity: Up to 0.08ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PPAR-gamma elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PPAR-gamma monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 17978516
• Molecular Weight: 21,580 Da
• NCBI Official Full Name: peroxisome proliferator activated receptor gamma
• UniProt Protein Name: Peroxisome proliferator-activated receptor gamma
• UniProt Gene Name: PPARG
• UniProt Synonym Gene Names: NR1C3; PPAR-gamma
• UniProt Entry Name: PPARG_HUMAN

Uniprot Description

PPAR-gamma: a transcription factor, member of the nuclear hormone receptor superfamily. Receptor for hypolipidemic drugs and fatty acids. Preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Regulator of adipogenesis and lipid metabolism, modulates insulin sensitivity, cell proliferation and inflammation. Phosphorylated and inhibited by MAP kinase. Heterodimerizes with the retinoid X receptor. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; nucleus; cytosol

Molecular Function: ligand-dependent nuclear receptor activity; transcription activator binding; zinc ion binding; drug binding; alpha-actinin binding; protein phosphatase binding; arachidonic acid binding; retinoid X receptor binding; protein binding; enzyme binding; DNA binding; ligand-dependent nuclear receptor transcription coactivator activity; prostaglandin receptor activity; sequence-specific DNA binding; steroid hormone receptor activity; estrogen receptor binding; chromatin binding; transcription factor activity

Biological Process: heart development; negative regulation of collagen biosynthetic process; positive regulation of transcription, DNA-dependent; cell maturation; rhythmic process; lipid homeostasis; glucose homeostasis; response to lipid; response to vitamin A; response to caffeine; positive regulation of oligodendrocyte differentiation; placenta development; long-chain fatty acid transport; organ regeneration; cell fate commitment; monocyte differentiation; negative regulation of acute inflammatory response; regulation of circadian rhythm; response to starvation; negative regulation of telomerase activity; cellular response to insulin stimulus; response to estrogen stimulus; response to mechanical stimulus; lipoprotein transport; brown fat cell differentiation; positive regulation of fat cell differentiation; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; fatty acid oxidation; positive regulation of transcription factor activity; positive regulation of phagocytosis, engulfment; negative regulation of transcription, DNA-dependent; negative regulation of smooth muscle cell proliferation; low-density lipoprotein receptor biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; epithelial cell differentiation; regulation of blood pressure; response to nutrient; caspase activation; transcription initiation from RNA polymerase II promoter; response to retinoic acid; G-protein coupled receptor protein signaling pathway; response to low density lipoprotein stimulus; positive regulation of fatty acid oxidation; white fat cell differentiation; innate immune response; gene expression; response to cold; negative regulation of cell growth; lipid metabolic process

Disease: Obesity; Carotid Intimal Medial Thickness 1; Lipodystrophy, Familial Partial, Type 3; Diabetes Mellitus, Noninsulin-dependent