Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Peroxisome proliferative activated receptor gama coactivator 1 alpha (PPARGC1) Standard Curve.)
Human Peroxisome Proliferative Activated Receptor gamma Coactivator 1 alpha (PPARGC1) ELISA Kit | PPARGC1 elisa kit
Human Peroxisome Proliferative Activated Receptor gamma Coactivator 1 alpha (PPARGC1) ELISA Kit
Gene Names
PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha)
Reactivity
Human
Synonyms
Peroxisome Proliferative Activated Receptor gamma Coactivator 1 alpha (PPARGC1); Human Peroxisome Proliferative Activated Receptor gamma Coactivator 1 alpha (PPARGC1) ELISA Kit; PPARGC1A; LEM6; PGC-1(alpha); PGC-1v; PGC1; PGC1A; PPARGC1; PPAR gamma coactivator variant form; PPAR gamma coactivator-1; ligand effect modulator-6; peroxisome proliferative activated receptor; gamma; coactivat; PPARGC1 elisa kit
Reactivity
Human
Specificity
Human Peroxisome proliferative activated receptor gama coactivator 1 alpha (PPARGC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PPARGC1A. No significant cross-reactivity or interference between Human PPARGC1A and analogues was observed.
Sequence Length
798
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Peroxisome proliferative activated receptor gama coactivator 1 alpha (PPARGC1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Peroxisome proliferative activated receptor gama coactivator 1 alpha (PPARGC1) Standard Curve.)
Related Product Information for PPARGC1 elisa kit
Description: This Human Peroxisome proliferative activated receptor gama coactivator 1 alpha (PPARGC1) ELISA Kit employs a two-site sandwich ELISA to quantitate PPARGC1A in samples. An antibody specific for PPARGC1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPARGC1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPARGC1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPARGC1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PPARG coactivator 1 alpha encoded by PPARGC1 is a transcriptional coactivator that regulates the genes involved in energy metabolism. PPARG coactivator 1 alpha interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. PPARG coactivator 1 alpha can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. PPARG coactivator 1 alpha may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.
Background: PPARG coactivator 1 alpha encoded by PPARGC1 is a transcriptional coactivator that regulates the genes involved in energy metabolism. PPARG coactivator 1 alpha interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. PPARG coactivator 1 alpha can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. PPARG coactivator 1 alpha may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
peroxisome proliferator-activated receptor gamma coactivator 1-alpha isoform 2
NCBI Official Synonym Full Names
PPARG coactivator 1 alpha
NCBI Official Symbol
PPARGC1A
NCBI Official Synonym Symbols
LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha)
NCBI Protein Information
peroxisome proliferator-activated receptor gamma coactivator 1-alpha
UniProt Protein Name
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
UniProt Gene Name
PPARGC1A
UniProt Synonym Gene Names
LEM6; PGC1; PGC1A; PPARGC1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha
UniProt Entry Name
PRGC1_HUMAN
NCBI Description
The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]
Research Articles on PPARGC1
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Product Notes
The Human PPARGC1 ppargc1a (Catalog #AAA9714860) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714860 ELISA Kit recognizes Human PPARGC1. It is sometimes possible for the material contained within the vial of "Peroxisome Proliferative Activated Receptor gamma Coactivator 1 alpha (PPARGC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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