Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Peroxidasin homolog (PXDN) Standard Curve.)
Human Peroxidasin Homolog (PXDN) ELISA Kit | PXDN elisa kit
Human Peroxidasin Homolog (PXDN) ELISA Kit
Gene Names
PXDN; PXN; VPO; MG50; PRG2; ASGD7; COPOA; D2S448; D2S448E
Reactivity
Human
Synonyms
Peroxidasin Homolog (PXDN); Human Peroxidasin Homolog (PXDN) ELISA Kit; PXDN; D2S448; D2S448E; KIAA0230; MG50; PRG2; PXN; VPO; p53-responsive gene 2; peroxidasin; PXDN elisa kit
Reactivity
Human
Specificity
Human Peroxidasin homolog (PXDN) ELISA Kit has high sensitivity and excellent specificity for detection of Human PXDN. No significant cross-reactivity or interference between Human PXDN and analogues was observed.
Sequence Length
1479
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Peroxidasin homolog (PXDN) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Peroxidasin homolog (PXDN) Standard Curve.)
Related Product Information for PXDN elisa kit
Description: This Human Peroxidasin homolog (PXDN) ELISA Kit employs a two-site sandwich ELISA to quantitate PXDN in samples. An antibody specific for PXDN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PXDN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXDN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXDN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Drosophila peroxidasin is an extracellular matrix-associated peroxidase. It is expressed exclusively in hemocytes derived from head mesoderm at a very early stage of differentiation. Peroxidasin exists as a homotrimer with a unique hybrid structure that combines an enzymatically functional peroxidase domain with motifs that are typically found in extracellular matrix-associated proteins. It is a secreted protein that contains a secretory recognition sequence at its N terminus. Peroxidasin catalyzes hydrogen peroxide-driven radioiodination, oxidations, and the formation of dityrosine in vitro. It is also thought to function in extracellular matrix consolidation, phagocytosis, and defense.
Background: Drosophila peroxidasin is an extracellular matrix-associated peroxidase. It is expressed exclusively in hemocytes derived from head mesoderm at a very early stage of differentiation. Peroxidasin exists as a homotrimer with a unique hybrid structure that combines an enzymatically functional peroxidase domain with motifs that are typically found in extracellular matrix-associated proteins. It is a secreted protein that contains a secretory recognition sequence at its N terminus. Peroxidasin catalyzes hydrogen peroxide-driven radioiodination, oxidations, and the formation of dityrosine in vitro. It is also thought to function in extracellular matrix consolidation, phagocytosis, and defense.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
peroxidasin homolog
NCBI Official Synonym Full Names
peroxidasin
NCBI Official Symbol
PXDN
NCBI Official Synonym Symbols
PXN; VPO; MG50; PRG2; ASGD7; COPOA; D2S448; D2S448E
NCBI Protein Information
peroxidasin homolog
UniProt Protein Name
Peroxidasin homolog
UniProt Gene Name
PXDN
UniProt Synonym Gene Names
KIAA0230; MG50; PRG2; VPO; VPO1
NCBI Description
This gene encodes a heme-containing peroxidase that is secreted into the extracellular matrix. It is involved in extracellular matrix formation, and may function in the physiological and pathological fibrogenic response in fibrotic kidney. Mutations in this gene cause corneal opacification and other ocular anomalies, and also microphthalmia and anterior segment dysgenesis. [provided by RefSeq, Aug 2014]
Uniprot Description
Displays low peroxidase activity and is likely to participate in H2O2 metabolism and peroxidative reactions in the cardiovascular system. Plays a role in extracellular matrix formation.
Research Articles on PXDN
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Product Notes
The Human PXDN pxdn (Catalog #AAA9714697) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714697 ELISA Kit recognizes Human PXDN. It is sometimes possible for the material contained within the vial of "Peroxidasin Homolog (PXDN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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