Rat Peroxidasin Homolog (PXDN) ELISA Kit | PXDN elisa kit

Rat Peroxidasin Homolog (PXDN) ELISA Kit

Cat. Num. AAA2707044

• Gene Names: PXDN; PXN; VPO; MG50; PRG2; ASGD7; COPOA; D2S448; D2S448E
• Reactivity: Rat
• Synonyms: Peroxidasin Homolog (PXDN); Rat Peroxidasin Homolog (PXDN) ELISA Kit; PRG2; PXN; MG50; VPO; VPO1; Melanoma-associated antigen MG50; Vascular peroxidase 1; p53-responsive gene 2 protein; PXDN elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Peroxidasin Homolog (PXDN).; No significant cross-reactivity or interference between Peroxidasin Homolog (PXDN) and analogues was observed.
• Sequence Length: 1479

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxidasin Homolog (PXDN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxidasin Homolog (PXDN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PXDN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxidasin Homolog (PXDN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxidasin Homolog (PXDN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxidasin Homolog (PXDN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxidasin Homolog (PXDN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 109150416
• NCBI GeneID: 7837
• NCBI Accession #: NP_036425.1
• NCBI GenBank Nucleotide #: NM_012293.2
• UniProt Accession #: Q92626; Q4KMG2; A8QM65; D6W4Y0
• Molecular Weight: 80,329 Da
• NCBI Official Full Name: peroxidasin homolog
• NCBI Official Synonym Full Names: peroxidasin
• NCBI Official Symbol: PXDN
• NCBI Official Synonym Symbols: PXN; VPO; MG50; PRG2; ASGD7; COPOA; D2S448; D2S448E
• NCBI Protein Information: peroxidasin homolog
• UniProt Protein Name: Peroxidasin homolog
• UniProt Gene Name: PXDN
• UniProt Synonym Gene Names: KIAA0230; MG50; PRG2; VPO; VPO1

NCBI Description

This gene encodes a heme-containing peroxidase that is secreted into the extracellular matrix. It is involved in extracellular matrix formation, and may function in the physiological and pathological fibrogenic response in fibrotic kidney. Mutations in this gene cause corneal opacification and other ocular anomalies, and also microphthalmia and anterior segment dysgenesis. [provided by RefSeq, Aug 2014]

Uniprot Description

PXDN: Displays low peroxidase activity and is likely to participate in H(2)O(2) metabolism and peroxidative reactions in the cardiovascular system. Plays a role in extracellular matrix formation. Belongs to the peroxidase family. XPO subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.11.1.7; Oxidoreductase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p25.3

Cellular Component: endoplasmic reticulum; extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: extracellular matrix structural constituent; heme binding; interleukin-1 receptor antagonist activity; metal ion binding; peroxidase activity

Biological Process: extracellular matrix organization; hydrogen peroxide catabolic process; immune response; negative regulation of cytokine and chemokine mediated signaling pathway; response to oxidative stress

Disease: Corneal Opacification With Other Ocular Anomalies