Human Pentraxin 3, Long (PTX3) ELISA Kit | PTX3 elisa kit

Human Anti-Pentraxin 3, Long Antibody (Anti-PTX3) ELISA Kit

Cat. Num. AAA2707874

• Gene Names: PTX3; TSG-14; TNFAIP5
• Reactivity: Human
• Applications: ELISA
• Synonyms: Pentraxin 3; Long (PTX3); Human Anti-Pentraxin 3; Long Antibody (Anti-PTX3) ELISA Kit; Long; TNFAIP5; TSG-14; Pentaxin-Related Gene; Rapidly Induced By IL-1 Beta; Tumor Necrosis Factor Alpha-Induced Protein 5; Tumor necrosis factor-inducible gene 14 protein; PTX3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Anti-Pentraxin 3, Long Antibody (Anti-PTX3).; No significant cross-reactivity or interference between Anti-Pentraxin 3, Long Antibody (Anti-PTX3) and analogues was observed.
• Sequence Length: 381

• Applicable Applications for PTX3 elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma and other biological fluids
• Detection Range: 31.2-2,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 13.3pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Pentraxin 3, Long Antibody (Anti-PTX3) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Pentraxin 3, Long Antibody (Anti-PTX3) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Pentraxin 3, Long Antibody (Anti-PTX3) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Pentraxin 3, Long Antibody (Anti-PTX3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 167900484
• NCBI GeneID: 5806
• NCBI Accession #: NP_002843.2
• NCBI GenBank Nucleotide #: NP_002843.2
• UniProt Accession #: P26022
• NCBI Official Full Name: pentraxin-related protein PTX3
• NCBI Official Synonym Full Names: pentraxin 3
• NCBI Official Symbol: PTX3
• NCBI Official Synonym Symbols: TSG-14; TNFAIP5
• NCBI Protein Information: pentraxin-related protein PTX3
• UniProt Protein Name: Pentraxin-related protein PTX3
• Protein Family: Pentraxin-related protein
• UniProt Gene Name: PTX3
• UniProt Synonym Gene Names: TNFAIP5; TSG14; TNF alpha-induced protein 5; TSG-14
• UniProt Entry Name: PTX3_HUMAN

NCBI Description

This gene encodes a member of the pentraxin protein family. The expression of this protein is induced by inflammatory cytokines in response to inflammatory stimuli in several mesenchymal and epithelial cell types, particularly endothelial cells and mononuclear phagocytes. The protein promotes fibrocyte differentiation and is involved in regulating inflammation and complement activation. It also plays a role in angiogenesis and tissue remodeling. The protein serves as a biomarker for several inflammatory conditions. [provided by RefSeq, Jun 2016]

Uniprot Description

PTX3: Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self- components and female fertility.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q25

Cellular Component: extracellular space; extracellular region

Molecular Function: complement component C1q binding; zymosan binding; virion binding

Biological Process: positive regulation of nitric oxide biosynthetic process; negative regulation of virion penetration into host cell; positive regulation of phagocytosis; innate immune response; response to yeast; opsonization; inflammatory response