Human PCSK7 (Proprotein convertase subtilisin/kexin type 7) ELISA Kit | PCSK7 elisa kit

Human PCSK7 (Proprotein convertase subtilisin/kexin type 7) ELISA Kit

Cat. Num. AAA7607181

• Gene Names: PCSK7; LPC; PC7; PC8; SPC7
• Reactivity: Human
• Synonyms: PCSK7 (Proprotein convertase subtilisin/kexin type 7); Human PCSK7 (Proprotein convertase subtilisin/kexin type 7) ELISA Kit; PCSK7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PCSK7. No significant cross-reactivity or interference between PCSK7 and analogues was observed.
• Sequence Length: 785

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: <0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PCSK7 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PCSK7 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PCSK7 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PCSK7 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PCSK7 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PCSK7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PCSK7 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 20336248
• NCBI GeneID: 9159
• NCBI Accession #: NP_004707.2
• NCBI GenBank Nucleotide #: NM_004716.3
• UniProt Accession #: Q16549; Q3C1X1; Q53GM4; Q96FK8; Q9UL57; B0YJ60
• Molecular Weight: 86,247 Da
• NCBI Official Full Name: proprotein convertase subtilisin/kexin type 7 preproprotein
• NCBI Official Synonym Full Names: proprotein convertase subtilisin/kexin type 7
• NCBI Official Symbol: PCSK7
• NCBI Official Synonym Symbols: LPC; PC7; PC8; SPC7
• NCBI Protein Information: proprotein convertase subtilisin/kexin type 7
• UniProt Protein Name: Proprotein convertase subtilisin/kexin type 7
• Protein Family: Proprotein convertase subtilisin/kexin
• UniProt Gene Name: PCSK7
• UniProt Synonym Gene Names: LPC; PC7; PC8; SPC7; PC7; PC8; hPC8

NCBI Description

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It can process proalbumin and is thought to be responsible for the activation of HIV envelope glycoproteins gp160 and gp140. This gene has been implicated in the transcriptional regulation of housekeeping genes and plays a role in the regulation of iron metabolism. A t(11;14)(q23;q32) chromosome translocation associated with B-cell lymphoma occurs between this gene and its inverted counterpart. [provided by RefSeq, Feb 2014]

Uniprot Description

Serine endoprotease that processes various proproteins by cleavage at paired basic amino acids, recognizing the RXXX[KR]R consensus motif. Likely functions in the constitutive secretory pathway.