Chicken Macrophage inflammatory protein-3beta ELISA Kit | MIP-3beta elisa kit

Chicken Macrophage inflammatory protein-3beta ELISA Kit

Cat. Num. AAA744076

• Gene Names: CCL19; ELC; CKb11; MIP3B; MIP-3b; SCYA19
• Reactivity: Chicken
• Synonyms: Macrophage inflammatory protein-3beta; Chicken Macrophage inflammatory protein-3beta ELISA Kit; Chicken Macrophage inflammatory protein-3b ELISA Kit; MIP-3beta elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This MIP-3beta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken MIP-3beta. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MIP-3beta elisa kit

Principle of the Assay: MIP-3beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MIP-3beta antibody and an MIP-3beta-HRP conjugate. The assay sample and buffer are incubated together with MIP-3beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MIP-3beta concentration since MIP-3beta from samples and MIP-3beta-HRP conjugate compete for the anti-MIP-3beta antibody binding site. Since the number of sites is limited, as more sites are occupied by MIP-3beta from the sample, fewer sites are left to bind MIP-3beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIP-3beta concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MIP-3beta elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 1791003
• NCBI GeneID: 6363
• UniProt Accession #: Q6IBD6; Q99731
• Molecular Weight: 10,993 Da
• NCBI Official Full Name: macrophage inflammatory protein 3 beta
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 19
• NCBI Official Symbol: CCL19
• NCBI Official Synonym Symbols: ELC; CKb11; MIP3B; MIP-3b; SCYA19
• NCBI Protein Information: C-C motif chemokine 19; exodus-3; CK beta-11; MIP-3-beta; EBI1 ligand chemokine; EBI1-ligand chemokine; CC chemokine ligand 19; beta chemokine exodus-3; beta-chemokine exodus-3; small-inducible cytokine A19; macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; epstein-Barr virus-induced molecule 1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19
• UniProt Protein Name: CCL19 protein
• UniProt Gene Name: CCL19
• UniProt Entry Name: Q6IBD6_HUMAN

NCBI Description

This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq, Jul 2008]

Uniprot Description

CCL19: May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: extracellular space; extracellular region

Molecular Function: CCR chemokine receptor binding; chemokine receptor binding; chemokine activity; CCR10 chemokine receptor binding; CCR7 chemokine receptor binding

Biological Process: formation of immunological synapse; positive regulation of interleukin-12 production; positive regulation of dendritic cell antigen processing and presentation; cell maturation; positive regulation of JNK cascade; positive regulation of NF-kappaB import into nucleus; positive regulation of endocytosis; positive regulation of interleukin-1 beta secretion; positive regulation of receptor-mediated endocytosis; activation of JNK activity; dendritic cell chemotaxis; establishment of T cell polarity; positive regulation of T cell proliferation; cell communication; inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of chemotaxis; response to virus; positive regulation of phosphoinositide 3-kinase activity; positive regulation of tumor necrosis factor production; cellular calcium ion homeostasis; positive regulation of protein kinase B signaling cascade; myeloid dendritic cell chemotaxis; positive regulation of protein kinase activity; release of sequestered calcium ion into cytosol; T cell costimulation; immune response; positive regulation of T-helper 1 cell differentiation