Typical Testing Data/Standard Curve (for reference only)
Human PAF ELISA Kit | PAF elisa kit
Human PAF (Platelet Activating Factor) ELISA Kit
Gene Names
KIAA0101; L5; PAF; OEATC; PAF15; OEATC1; p15PAF; NS5ATP9; OEATC-1; p15/PAF; p15(PAF)
Reactivity
Human
Synonyms
PAF; Human PAF (Platelet Activating Factor) ELISA Kit; PAF elisa kit
Reactivity
Human
Specificity
This kit recognizes Human PAF in samples. No significant cross-reactivity or interference between Human PAF and analogues was observed.
Sequence Length
65
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
78.13-5000pg/mL
Sensitivity
46.88pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PAF were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PAF were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PAF elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PAF concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PAF. You can calculate the concentration of Human PAF in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PAF. You can calculate the concentration of Human PAF in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
7,221 Da
NCBI Official Full Name
PCNA-associated factor isoform 2
NCBI Official Synonym Full Names
KIAA0101
NCBI Official Symbol
KIAA0101
NCBI Official Synonym Symbols
L5; PAF; OEATC; PAF15; OEATC1; p15PAF; NS5ATP9; OEATC-1; p15/PAF; p15(PAF)
NCBI Protein Information
PCNA-associated factor; HCV NS5A-transactivated protein 9; PCNA-associated factor of 15 kDa; hepatitis C virus NS5A-transactivated protein 9; overexpressed in anaplastic thyroid carcinoma 1
UniProt Protein Name
PCNA-associated factor
Protein Family
UniProt Gene Name
KIAA0101
UniProt Synonym Gene Names
NS5ATP9; PAF; HCV NS5A-transactivated protein 9; OEATC-1; PAF15; p15PAF
UniProt Entry Name
PAF15_HUMAN
Uniprot Description
PAF: May be involved in protection of cells from UV-induced cell death.
Protein type: DNA repair, damage
Chromosomal Location of Human Ortholog: 15q22.31
Cellular Component: perinuclear region of cytoplasm; cytoplasm; nucleus
Molecular Function: protein binding; chromatin binding
Biological Process: bypass DNA synthesis; regulation of cell cycle; centrosome organization and biogenesis; DNA replication; response to DNA damage stimulus; response to UV
Research Articles on PAF
Similar Products
Product Notes
The Human PAF kiaa0101 (Catalog #AAA2514473) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2514473 ELISA Kit recognizes Human PAF. It is sometimes possible for the material contained within the vial of "PAF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
