Typical Testing Data/Standard Curve (for reference only)
Human Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) ELISA Kit | PNPLA3 elisa kit
Human Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) ELISA Kit
Gene Names
PNPLA3; ADPN; C22orf20; iPLA(2)epsilon
Reactivity
Human
Synonyms
Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3); Human Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) ELISA Kit; ADPN; C22orf20; iPLA(2)epsilon; Adiponutrin; Acylglycerol O-acyltransferase; Calcium-independent phospholipase A2-epsilon; PNPLA3 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of PLXND1. No significant cross-reactivity or interference between PLXND1 and analogues was observed.
Sequence Length
477
Samples
Human Tissue Homogenates, Cell Lysates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
0.119ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PLXND1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PLXND1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PNPLA3 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PLXND1 in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PLXND1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PLXND1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLXND1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PLXND1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PLXND1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PLXND1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLXND1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PLXND1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,485 Da
NCBI Official Full Name
patatin-like phospholipase domain-containing protein 3
NCBI Official Synonym Full Names
patatin like phospholipase domain containing 3
NCBI Official Symbol
PNPLA3
NCBI Official Synonym Symbols
ADPN; C22orf20; iPLA(2)epsilon
NCBI Protein Information
patatin-like phospholipase domain-containing protein 3
UniProt Protein Name
Patatin-like phospholipase domain-containing protein 3
Protein Family
UniProt Gene Name
PNPLA3
UniProt Synonym Gene Names
ADPN; C22orf20; iPLA2-epsilon
NCBI Description
The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. [provided by RefSeq, Jul 2008]
Uniprot Description
Multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities.
Research Articles on PNPLA3
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Product Notes
The Human PNPLA3 pnpla3 (Catalog #AAA2706527) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2706527 ELISA Kit recognizes Human PNPLA3. It is sometimes possible for the material contained within the vial of "Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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