Human Nucleoporin 62kDa (NUP62) ELISA Kit | NUP62 elisa kit

Human Nucleoporin 62kDa (NUP62) ELISA Kit

Cat. Num. AAA450709

• Gene Names: NUP62; p62; IBSN; SNDI
• Reactivity: Human
• Synonyms: Nucleoporin 62kDa (NUP62); Human Nucleoporin 62kDa (NUP62) ELISA Kit; NUP62 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NUP62. No significant cross-reactivity or interference between NUP62 and analogues was observed.
• Sequence Length: 522

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.112ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NUP62 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NUP62 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NUP62 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NUP62 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NUP62. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NUP62. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NUP62, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NUP62 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 301069416
• NCBI GeneID: 23636
• NCBI Accession #: NP_001180286.1
• NCBI GenBank Nucleotide #: NM_001193357.1
• UniProt Accession #: P37198; Q503A4; Q6GTM2; Q96C43; Q9NSL1; B3KWU5
• Molecular Weight: 53,255 Da
• NCBI Official Full Name: nuclear pore glycoprotein p62
• NCBI Official Synonym Full Names: nucleoporin 62
• NCBI Official Symbol: NUP62
• NCBI Official Synonym Symbols: p62; IBSN; SNDI
• NCBI Protein Information: nuclear pore glycoprotein p62
• UniProt Protein Name: Nuclear pore glycoprotein p62
• Protein Family: Nuclear pore glycoprotein
• UniProt Gene Name: NUP62

NCBI Description

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex.