Human Receptor II for the Fc region of immunoglobulin E ELISA Kit | FceRII/CD23 elisa kit

Human Receptor II for the Fc region of immunoglobulin E ELISA Kit

Cat. Num. AAA753460

• Gene Names: FCER2; CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
• Reactivity: Human
• Synonyms: Receptor II for the Fc region of immunoglobulin E; Human Receptor II for the Fc region of immunoglobulin E ELISA Kit; FceRII/CD23 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FceRII/CD23 elisa kit

Intended Uses: This FceRII ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FceRII.

Principle of the Assay: FceRII ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-FceRII antibody and an FceRII-HRP conjugate. The assay sample and buffer are incubated together with FceRII-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FceRII concentration since FceRII from samples and FceRII-HRP conjugate compete for the anti-FceRII antibody binding site. Since the number of sites is limited, as more sites are occupied by FceRII from the sample, fewer sites are left to bind FceRII-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FceRII concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FceRII/CD23 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 334085205
• NCBI GeneID: 2208
• NCBI Accession #: NP_001193948.2
• NCBI GenBank Nucleotide #: NM_001207019.2
• Molecular Weight: 36,469 Da
• NCBI Official Full Name: low affinity immunoglobulin epsilon Fc receptor isoform b
• NCBI Official Synonym Full Names: Fc fragment of IgE, low affinity II, receptor for (CD23)
• NCBI Official Symbol: FCER2
• NCBI Official Synonym Symbols: CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2
• NCBI Protein Information: low affinity immunoglobulin epsilon Fc receptor; CD23 antigen; fc-epsilon-RII; lymphocyte IgE receptor; immunoglobulin E-binding factor; C-type lectin domain family 4, member J
• UniProt Protein Name: Low affinity immunoglobulin epsilon Fc receptor
• UniProt Gene Name: FCER2
• UniProt Synonym Gene Names: CD23A; CLEC4J; FCE2; IGEBF
• UniProt Entry Name: FCER2_HUMAN

NCBI Description

The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).