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Typical Testing Data/Standard Curve (for reference only)

Human NT ELISA Kit | NT elisa kit

Human NT (Neurotensin) ELISA Kit

Gene Names
NTS; NN; NT; NT/N; NTS1; NMN-125
Reactivity
Human
Synonyms
NT; Human NT (Neurotensin) ELISA Kit; NT elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This kit recognizes Human NT in samples. No significant cross-reactivity or interference between Human NT and analogues was observed.
Sequence Length
170
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Competitive
Detection Range
78.13-5000pg/mL
Sensitivity
46.88pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human NT were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human NT were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NT elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human NT concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human NT. During the reaction, Human NT in the sample or standard competes with a fixed amount of Human NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human NT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Human NT in the samples is then determined by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19,795 Da
NCBI Official Full Name
neurotensin/neuromedin N preproprotein
NCBI Official Synonym Full Names
neurotensin
NCBI Official Symbol
NTS
NCBI Official Synonym Symbols
NN; NT; NT/N; NTS1; NMN-125
NCBI Protein Information
neurotensin/neuromedin N; pro-neurotensin/neuromedin
UniProt Protein Name
Neurotensin/neuromedin N
Protein Family
UniProt Gene Name
NTS
UniProt Synonym Gene Names
NN; NmN; NT
UniProt Entry Name
NEUT_HUMAN

NCBI Description

This gene encodes a common precursor for two peptides, neuromedin N and neurotensin. Neurotensin is a secreted tridecapeptide, which is widely distributed throughout the central nervous system, and may function as a neurotransmitter or a neuromodulator. It may be involved in dopamine-associated pathophysiological events, in the maintenance of gut structure and function, and in the regulation of fat metabolism. Neurotensin also exhibits antimicrobial activity against bacteria and fungi. Tissue-specific processing may lead to the formation in some tissues of larger forms of neuromedin N and neurotensin. The large forms may represent more stable peptides that are also biologically active. [provided by RefSeq, Oct 2014]

Uniprot Description

NTS: Neurotensin may play an endocrine or paracrine role in the regulation of fat metabolism. It causes contraction of smooth muscle. Belongs to the neurotensin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q21

Cellular Component: transport vesicle; extracellular region

Molecular Function: neuropeptide hormone activity; protein binding

Biological Process: regulation of blood vessel size; signal transduction

Research Articles on NT

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Product Notes

The Human NT nts (Catalog #AAA2506296) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2506296 ELISA Kit recognizes Human NT. It is sometimes possible for the material contained within the vial of "NT, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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