Typical Testing Data/Standard Curve (for reference only)
Human NT-proBNP ELISA Kit | NT-proBNP elisa kit
NT-proBNP Human ELISA Kit
Reactivity
Human
Applications
ELISA
Synonyms
NT-proBNP; NT-proBNP Human ELISA Kit; Human NT-proBNP; NT-proBNP elisa kit
Reactivity
Human
Applicable Applications for NT-proBNP elisa kit
Quantitative sELISA (EIA)
Application Notes
For quantitative detection of human NT-proBNP in serum, plasma and cell culture supernatants.
Samples
Serum, plasma and cell culture supernatants
Assay Type
Quantitative Sandwich
Detection Range
62.5 pg/ml - 4000 pg/ml
Sensitivity
2 pg/ml
Preparation and Storage
Store at -20 degree C for one year.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NT-proBNP elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-NT-proBNP polyclonal antibody was pre-coated onto 96-well plates. The standards and test samples were added to the wells and any NT-proBNP present is bound by the immobilized antibody. After washing away any unbound substances, the HRP conjugated anti-NT-proBNP monoclonal antibody was added to wells as detection antibodies. Following a wash to remove any unbound antibody-enzyme reagent, the TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding stop solution. The density of yellow is proportional to the NT-proBNP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NT-proBNP can be calculated.
Background/Introduction: The N-terminal prohormone of brain natriuretic peptide (NT-proBNP) is a 76 amino acid N-terminal fragment of brain natriuretic peptide. Both BNP and NT-proBNP levels in the blood are used for screening, identification of acute congestive heart failure (CHF) and may be useful to establish prognosis in heart failure, as both markers are typically higher in patients with worse outcome.BNP may be a useful screening tool for left ventricular dysfunction in patients with history suggestive of heart disease and be used to assist in forming a pretest probability, which in turn could greatly assist in appropriateness of patient referral and in optimization of drug therapy.
Background/Introduction: The N-terminal prohormone of brain natriuretic peptide (NT-proBNP) is a 76 amino acid N-terminal fragment of brain natriuretic peptide. Both BNP and NT-proBNP levels in the blood are used for screening, identification of acute congestive heart failure (CHF) and may be useful to establish prognosis in heart failure, as both markers are typically higher in patients with worse outcome.BNP may be a useful screening tool for left ventricular dysfunction in patients with history suggestive of heart disease and be used to assist in forming a pretest probability, which in turn could greatly assist in appropriateness of patient referral and in optimization of drug therapy.
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Product Notes
The Human NT-proBNP (Catalog #AAA355233) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA355233 ELISA Kit recognizes Human NT-proBNP. AAA Biotech's NT-proBNP can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA). For quantitative detection of human NT-proBNP in serum, plasma and cell culture supernatants. Researchers should empirically determine the suitability of the NT-proBNP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NT-proBNP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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