Human NOX5 ELISA Kit | NOX5 elisa kit

Human NOX5 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5) ELISA Kit

Cat. Num. AAA2512643

• Reactivity: Human
• Synonyms: NOX5; Human NOX5 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 5) ELISA Kit; NOX5 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human NOX5 in samples. No significant cross-reactivity or interference between Human NOX5 and analogues was observed.
• Sequence Length: 765

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 18.75pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human NOX5 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human NOX5 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOX5 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human NOX5 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NOX5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOX5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOX5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human NOX5. You can calculate the concentration of Human NOX5 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 296278229
• NCBI GeneID: 79400
• NCBI Accession #: NP_078781.3
• NCBI GenBank Nucleotide #: NM_024505.3
• UniProt Accession #: Q96PH1; Q08AN2; Q08AN3; Q8TEQ1; Q8TER4; Q96PH2; Q96PJ8; Q96PJ9; Q9H6E0; Q9HAM8; B2RBJ4
• Molecular Weight: 83,109 Da
• NCBI Official Full Name: NADPH oxidase 5 isoform 1
• NCBI Official Synonym Full Names: NADPH oxidase, EF-hand calcium binding domain 5
• NCBI Official Symbol: NOX5
• NCBI Protein Information: NADPH oxidase 5
• UniProt Protein Name: NADPH oxidase 5
• Protein Family: NADPH oxidase
• UniProt Gene Name: NOX5
• UniProt Entry Name: NOX5_HUMAN

NCBI Description

This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

NOX5: Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Oxidoreductase; Membrane protein, multi-pass; EC 1.6.3.-

Chromosomal Location of Human Ortholog: 15q23

Cellular Component: endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; FAD binding; superoxide-generating NADPH oxidase activity; heme binding; calcium ion binding; hydrogen ion channel activity; NADP binding

Biological Process: cell proliferation; proton transport; regulation of fusion of sperm to egg plasma membrane; apoptosis; endothelial cell proliferation; regulation of proton transport; cytokinesis; angiogenesis; superoxide release; cytokine secretion